Christensson P I, Stenram U
Ups J Med Sci. 1980;85(1):19-27. doi: 10.3109/03009738009179168.
Rats were starved for 7 days or fed a protein-free or a 25% casein diet for 7 or 14 days. Free and total ribosomes were prepared from the liver. A determination of the ratio of mg RNA of ribosomes to mg DNA of the total homogenate was made. In starved rats, RNA of free and membrane-bound ribosomes decreased to the same extent. In rats fed a protein-free diet only mg RNA of membrane-bound ribosomes decreased significantly, and after 14 days the ratio of RNA of free to total ribosomes was increased. NADPH-cytochrome c reductase activity in the liver decreased per mg DNA in protein deprivation but was unchanged per mg RNA of membrane-bound ribosomes.
将大鼠饥饿7天，或给予无蛋白饮食或25%酪蛋白饮食7天或14天。从肝脏中制备游离核糖体和总核糖体。测定核糖体的RNA毫克数与全匀浆DNA毫克数的比值。在饥饿的大鼠中，游离核糖体和膜结合核糖体的RNA下降程度相同。在给予无蛋白饮食的大鼠中，仅膜结合核糖体的RNA毫克数显著下降，14天后游离核糖体与总核糖体的RNA比值增加。蛋白质缺乏时，肝脏中每毫克DNA的NADPH-细胞色素c还原酶活性下降，但每毫克膜结合核糖体的RNA该酶活性不变。
