Ramsey J C, Steele W J
Biochim Biophys Acta. 1976 Oct 18;447(3):312-8. doi: 10.1016/0005-2787(76)90054-x.
The distribution of free and membrane-bound ribosomes in liver in response to starvation has not been clearly defined. An investigation has been made of the effects of starvation on the content of DNA, RNA, protein, phospholipid and glycogen in rat liver, on the distribution of free and membrane-bound ribosomes, and on the content of phospholipid and glycogen in free and bound ribosome fractions. The results indicate that starvation can produce up to a 50% reduction in hepatic ribosomes without altering either the fraction of rRNA relative to the total RNA or the distribution of free and membrane-bound ribosomes. In addition, the degree of contamination of isolated ribosomes with membranous material does not fluctuate with changes in the nutritional status of the animal. The results suggest that the relative capacities for protein synthesis among the two ribosome compartments are maintained during the early stages of starvation. Further, co-sedimentation with glycogen is not responsible for the presence of membranous materials in purified ribosomes.
饥饿状态下肝脏中游离核糖体和膜结合核糖体的分布尚未明确界定。本研究调查了饥饿对大鼠肝脏中DNA、RNA、蛋白质、磷脂和糖原含量的影响，对游离核糖体和膜结合核糖体分布的影响，以及对游离核糖体和结合核糖体组分中磷脂和糖原含量的影响。结果表明，饥饿可使肝脏核糖体减少多达50%，而不会改变rRNA相对于总RNA的比例，也不会改变游离核糖体和膜结合核糖体的分布。此外，分离的核糖体被膜性物质污染的程度不会随动物营养状态的变化而波动。结果表明，在饥饿早期，两个核糖体区室之间的蛋白质合成相对能力得以维持。此外，与糖原的共沉降并不是纯化核糖体中存在膜性物质的原因。
