Mansbach C M
Am J Dig Dis. 1978 Jun;23(6):486-92. doi: 10.1007/BF01072691.
Cryptal lecithin-synthesizing enzyme specific activities have been previously demonstrated to be increased by feeding a fat-supplemented diet to hamsters. To determine if a physiological basis exists for such changes [3H]thymidine incorporation, cellular migration rate, and mucosal concentration of DNA, protein, and lecithin were measured. Radioautographic studies showed that the labeling index and cell migration rate throughout the intestine in the fat-fed hamsters and in the proximal three-quarters of the intestine of the control group were the same. Both parameters were reduced in the distal quarter of the control intestine. The protein/DNA ratio was significantly increased in the proximal three-quarters and modestly in the distal quarter of the intestine of the fat-fed group as compared to controls, suggesting cellular hypertrophy. The lecithin content of the proximal three-quarters of intestine was the same in both groups but reduced in the distal quarter of the gut of the fat-fed group. These studies suggest that lipid feeding in the hamster can have profound effects on intestinal cellular content and turnover.
先前已证明，给仓鼠喂食高脂肪饮食会增加其隐窝卵磷脂合成酶的比活性。为了确定这种变化是否存在生理基础，我们测量了[3H]胸腺嘧啶核苷掺入量、细胞迁移率以及DNA、蛋白质和卵磷脂的黏膜浓度。放射自显影研究表明，喂食脂肪的仓鼠整个肠道的标记指数和细胞迁移率，与对照组近端四分之三肠道的标记指数和细胞迁移率相同。在对照组肠道的远端四分之一，这两个参数均降低。与对照组相比，喂食脂肪组肠道近端四分之三的蛋白质/DNA比值显著增加，远端四分之一则略有增加，提示细胞肥大。两组近端四分之三肠道的卵磷脂含量相同，但喂食脂肪组肠道远端四分之一的卵磷脂含量降低。这些研究表明，给仓鼠喂食脂质会对肠道细胞含量和更新产生深远影响。
