枯草芽孢杆菌起始tRNA的核苷酸序列。
Nucleotide sequence of initiator tRNA from Bacillus subtilis.
作者信息
Yamada Y, Kuchino Y, Ishikura H
出版信息
J Biochem. 1980 May;87(5):1261-9. doi: 10.1093/oxfordjournals.jbchem.a132863.
Initiator methionine tRNA, tRNAfMet, was purified from Bacillus subtilis W168 by the use of two column chromatographies on DEAE-Sephadex A-50 and BD-cellulose. The nucleotide sequence was determined to be pC-G-C-G-G-G-G-U-G-G-A-G-C-A-G-U-U-C-G-G-D-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A-C-C-C-G-A-A-G-G-U-C-G-C-A-G-G-T-psi-C-A-A-A-U-C-C-U-G-C-C-C-C-C-G-C-A-A-C-C-AOH. This TRNAfMet exhibited a melting temperature 8 degrees C lower than that of E. coli tRNAfMet in the presence of 0.01 M magnesium acetate.
起始甲硫氨酸tRNA，即tRNAfMet，通过在DEAE-葡聚糖A-50和BD-纤维素上进行两次柱色谱法从枯草芽孢杆菌W168中纯化得到。其核苷酸序列测定为pC-G-C-G-G-G-G-U-G-G-A-G-C-A-G-U-U-C-G-G-D-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A-C-C-C-G-A-A-G-G-U-C-G-C-A-G-G-T-ψ-C-A-A-A-U-C-C-U-G-C-C-C-C-C-G-C-A-A-C-C-AOH。在存在0.01 M乙酸镁的情况下，这种tRNAfMet的解链温度比大肠杆菌tRNAfMet的解链温度低8摄氏度。
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