枯草芽孢杆菌赖氨酸转运RNA的核苷酸序列。
Nucleotide sequence of a lysine tRNA from Bacillus subtilis.
作者信息
Yamada Y, Ishikura H
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4291-303. doi: 10.1093/nar/4.12.4291.
Abstract
A lysine tRNA (tRNA1Lys) was purified from Bacillus subtilis W168 by a consecutive use of several column chromatographic systems. The nucleotide sequence was determined to be pG-A-G-C-C-A-U-U-A-G-C-U-C-A-G-U-D-G-G-D-A-G-A-G-C-A-U-C-U-G-A-C-U-U(U*)-U-U-K-A-psi-C-A-G-A-G-G-m7G(G)-U-C-G-A-A-G-G-T-psi-C-G-A-G-U-C-C-U-U-C-A-U-G-G-C-U-C-A-C-C-AOH, where K and U* are unidentified nucleosides. The nucleosides of U34 and m7G46 were partially substituted with U* and G, respectively. The binding ability of lysyl-tRNA1Lys to Escherichia coli ribosomes was stimulated with ApApA as well as ApApG.
摘要
通过连续使用多种柱色谱系统从枯草芽孢杆菌W168中纯化出一种赖氨酸tRNA（tRNA1Lys）。测定其核苷酸序列为pG-A-G-C-C-A-U-U-A-G-C-U-C-A-G-U-D-G-G-D-A-G-A-G-C-A-U-C-U-G-A-C-U-U(U*)-U-U-K-A-psi-C-A-G-A-G-G-m7G(G)-U-C-G-A-A-G-G-T-psi-C-G-A-G-U-C-C-U-U-C-A-U-G-G-C-U-C-A-C-C-AOH，其中K和U为未鉴定的核苷。U34和m7G46的核苷分别部分被U和G取代。ApApA以及ApApG均可刺激赖氨酰-tRNA1Lys与大肠杆菌核糖体的结合能力。
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