Murao K, Hasegawa T, Ishikura H
Nucleic Acids Res. 1982 Jan 22;10(2):715-8. doi: 10.1093/nar/10.2.715.
A valine tRNA was purified from Bacillus subtilis W168 by a combined use of several column chromatographic systems. The nucleotide sequence was determined to be pG-G-A-G-G-A-U-U-A-G-C-U-C-A-G-C-D-G-G-G-A-G-A-G-C-A-U-C--G-C-C-U-mo5U-A-C-m6-A -A-G-C-A-G-A-G-G-m7G-U-C-G-G-C-G-G-T-psi-C-G-A-G-C-C-C-G-U-C-A-U-C-C-U-C-C-A-C- C-AOH with the main use of of non-labeled tRNA and with the subsidiary use of [32P]-post-labeled sample. This tRNA contains 5-methoxy-uridine (mo5U) at the wobble position of the anticodon. A binding experiment of valyl-tRNA to ribosome revealed that mo5U is recognized by A and G, and fairly well by U.
通过联合使用多种柱色谱系统，从枯草芽孢杆菌W168中纯化出一种缬氨酸tRNA。使用未标记的tRNA为主，并辅助使用[32P]后标记样品，确定其核苷酸序列为pG-G-A-G-G-A-U-U-A-G-C-U-C-A-G-C-D-G-G-G-A-G-A-G-C-A-U-C--G-C-C-U-mo5U-A-C-m6-A -A-G-C-A-G-A-G-G-m7G-U-C-G-G-C-G-G-T-psi-C-G-A-G-C-C-C-G-U-C-A-U-C-C-U-C-C-A-C- C-AOH。该tRNA在反密码子的摆动位置含有5-甲氧基尿苷（mo5U）。缬氨酰-tRNA与核糖体的结合实验表明，mo5U可被A和G识别，也能被U较好地识别。
