Csaba G, Bukta A, Kovács P
Acta Anat (Basel). 1977;98(2):154-61. doi: 10.1159/000144790.
Having applied the picric acid-bromphenol blue (BPB) and the phosphomolybdic acid-benzidine (PMA-B) methods--the first method detecting the total histone content, the second one only free histones--we have found that cell nuclei of the rat embryos permanently contain histones, nevertheless, in such a pattern that the free histone appears only at a well-defined stage of embryogenesis. Strong PMA-B reaction is observable in the nuclei of some mesenchymal cells and in the reorganizing somites' cell nuclei of the 13-day-old embryo as well as in the hemopoietic elements of the 15- and 17-day old embryos' liver. Within nuclei of the red blood cells of the 13-day-old embryo an extremely intense reaction is seen which then disappears till the 15th day, while the BPB reaction shows a gradual intensification up to the neonatal age. The experiments support the former model of one of the authors for a histone-nonhistone control of ontogenesis.
应用苦味酸-溴酚蓝（BPB）法和磷钼酸-联苯胺（PMA-B）法——第一种方法检测总组蛋白含量，第二种方法仅检测游离组蛋白——我们发现大鼠胚胎细胞核中始终含有组蛋白，不过，游离组蛋白仅在胚胎发育的特定阶段出现。在13日龄胚胎的一些间充质细胞核以及正在重组的体节细胞核中，以及在15日龄和17日龄胚胎肝脏的造血细胞中，可观察到强烈的PMA-B反应。在13日龄胚胎红细胞的细胞核内可见极其强烈的反应，该反应随后直至第15天消失，而BPB反应则显示直至新生儿期逐渐增强。这些实验支持了作者之一先前提出的关于组蛋白-非组蛋白对个体发育控制的模型。
