Maki R, Kearney J, Paige C, Tonegawa S
Science. 1980 Sep 19;209(4463):1366-9. doi: 10.1126/science.6774416.
Two types of immature B cells, namely fetal liver hybridomas and the leukemic cell line 70Z/3, both of which have cytoplasmic mu chains but no light chains, were examined for DNA rearrangements of their light chain and heavy chain immunoglobulin genes. In the fetal liver hybridomas, which were constructed from fetal liver cells and a tumor cell, no light chain gene rearrangement was observed, whereas in the 70Z/3 cell line a kappa light chain rearrangement probably occurred. The results suggest that, although the lack of light chain synthesis can be due to a lack of gene rearrangement, there may also be transcriptional regulation, which may also be important for the expression of light chain immunoglobulins in immature B cells.
两种未成熟B细胞，即胎肝杂交瘤和白血病细胞系70Z/3，二者均有细胞质μ链但无轻链，对其轻链和重链免疫球蛋白基因的DNA重排进行了检测。在由胎肝细胞和肿瘤细胞构建的胎肝杂交瘤中，未观察到轻链基因重排，而在70Z/3细胞系中可能发生了κ轻链重排。结果表明，虽然轻链合成的缺乏可能是由于基因重排的缺失，但也可能存在转录调控，这对于未成熟B细胞中轻链免疫球蛋白的表达可能也很重要。
