常规性病实验室中淋病奈瑟菌的鉴定：协同凝集、直接免疫荧光和糖发酵反应的比较研究
Identification of Neisseria gonorrhoeae in the routine venerelogical laboratory: Comparative study of coagglutination, direct immunofluorescence, and sugar fermentation reaction.
作者信息
Rufli T
出版信息
Br J Vener Dis. 1980 Jun;56(3):144-7. doi: 10.1136/sti.56.3.144.
Abstract
The coagglutination (CoA) method for the identification of Neisseria gonorrhoeae colonies grown on selective culture media was used on 116 strains in a routine venereological laboratory together with the direct immunofluorescence (IF) test and the sugar fermentation reaction. Correlation of results between the CoA method and the direct IF test and between the CoA test and the sugar fermentation reaction was 95.7% and 97% respectively. The sugar fermentation reaction requires subcultures and is more time-consuming than the IF test; the latter needs elaborate technical equipment and experience. The CoA method however can be carried out with the primary culture, is technically easy to perform and to reproduce, and the result is available within minutes.
摘要
在一家常规性病实验室，对116株在选择性培养基上生长的淋病奈瑟菌菌落采用协同凝集（CoA）法进行鉴定，并与直接免疫荧光（IF）试验及糖发酵反应一起使用。CoA法与直接IF试验之间以及CoA试验与糖发酵反应之间的结果相关性分别为95.7%和97%。糖发酵反应需要传代培养，比IF试验耗时更长；后者需要精密的技术设备和经验。然而，CoA法可在原代培养时进行，技术操作简便且可重复，几分钟内即可得出结果。
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