用于初代培养物中淋病奈瑟菌鉴定的协同凝集试验评估
Evaluation of the coagglutination test for the identification of Neisseria gonorrhoeae in primary cultures.
作者信息
Johnston N A
出版信息
Br J Vener Dis. 1981 Oct;57(5):315-9. doi: 10.1136/sti.57.5.315.
Abstract
The coagglutination (COA) test for the identification of Neisseria gonorrhoeae was compared with immunofluorescence and sugar degradation tests on 1710 gonococcal isolates, 72 of which produce beta-lactamase. The COA test gave a positive result for 98.6% of the strains. Treatment of suspensions with Streptomyces enzyme reduced the incidence of inconclusive results due to autoagglutination to 1.2%. Cross-reactivity of the gonococcal antiserum was minimised by absorption with meningococci and Moraxella species. The COA provide a simple, quick, and reliable method for identifying N gonorrhoeae in culture.
摘要
采用协同凝集试验（COA）对淋病奈瑟菌进行鉴定，并与免疫荧光法和糖降解试验对1710株淋球菌分离株进行比较，其中72株产生β-内酰胺酶。COA试验对98.6%的菌株呈阳性结果。用链霉菌酶处理菌悬液可将因自身凝集导致的不确定结果发生率降至1.2%。用脑膜炎球菌和莫拉菌属进行吸收，可使淋球菌抗血清的交叉反应性降至最低。COA为培养物中淋病奈瑟菌的鉴定提供了一种简单、快速且可靠的方法。
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本文引用的文献
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NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.淋病奈瑟菌。一、与克隆变异基因连锁的毒力。
J Bacteriol. 1963 Jun;85(6):1274-9. doi: 10.1128/jb.85.6.1274-1279.1963.
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