Cavagnaro J, Pierce D A, Lucchesi J C, Chae C B
J Cell Biol. 1980 Nov;87(2 Pt 1):415-9. doi: 10.1083/jcb.87.2.415.
Incubation of Drosophila salivary glands with radioactive diisopropyl fluorophosphate results in the uniform labeling of polytene chromosomes. Extensive labeling is seen only when chromosome squashes are prepared by a formaldehyde fixation procedure and not by standard acetic acid techniques. The labeling is inhibited in the presence of tosylphenylalanine chloromethyl ketone and phenylmethane sulfonylfluoride but not by tosyllysine chloromethyl ketone, suggesting that a chymotrypsin-like serine protease is associated with the chromosomes. Protease inhibitors show no apparent effect on heat-shock specific puffing.
用放射性二异丙基氟磷酸酯处理果蝇唾液腺会导致多线染色体均匀标记。只有当通过甲醛固定程序制备染色体压片而非标准醋酸技术时,才会看到广泛的标记。在甲苯磺酰苯丙氨酸氯甲基酮和苯甲基磺酰氟存在时标记受到抑制,但甲苯磺酰赖氨酸氯甲基酮则无此作用,这表明一种类胰凝乳蛋白酶的丝氨酸蛋白酶与染色体相关。蛋白酶抑制剂对热休克特异性胀泡没有明显影响。