Dudler R, Egg A H, Kubli E, Artavanis-Tsakonas S, Gehring W J, Steward R, Schedl P
Nucleic Acids Res. 1980 Jul 11;8(13):2921-37. doi: 10.1093/nar/8.13.2921.
Three recombinant plasmids containing randomly sheared genomic D. melanogaster tRNAs have been identified and characterized in detail. One of these, the plasmid 14C4, has a D. melanogaster (Dm) DNA segment of 18 kb, and has three tRNA2Arg and two tRNAAsN genes. The second plasmid, 38B10, has tRNAHis genes, while the third plasmid, 63H5, contains coding sequences for tRNA2Asp. The Dm DNA segments in each recombinant plasmid are derived from unique cytogenetic loci. 14C4 is from 84 F, 38B10 is from 48 F and 63H5 is from 70 A.
已鉴定并详细表征了三个含有随机剪切的黑腹果蝇基因组tRNA的重组质粒。其中之一,即质粒14C4,有一个18 kb的黑腹果蝇(Dm)DNA片段,含有三个tRNA2Arg基因和两个tRNAAsN基因。第二个质粒38B10含有tRNAHis基因,而第三个质粒63H5含有tRNA2Asp的编码序列。每个重组质粒中的Dm DNA片段均来自独特的细胞遗传学位点。14C4来自84 F,38B10来自48 F,63H5来自70 A。