Department of Chemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406.
Plant Physiol. 1990 Apr;92(4):939-45. doi: 10.1104/pp.92.4.939.
DNA polymerases were purified from chloroplasts and mitochondria of cultured Glycine max cells. The chloroplast enzyme exists in two forms which are indistinguishable from each other biochemically. All three organellar enzymes have an estimated molecular weight of 85,000 to 90,000 and prefer poly(rA)dT(12-18) over activated DNA as a template in vitro. Maximum activity of the chloroplast and mitochondrial DNA polymerases requires KCl and a reducing agent, and the enzymes are completely resistant to inhibitors of DNA polymerase alpha. Taken together, these properties classify the soybean organellar enzymes as DNA polymerases gamma. A unique feature that distinguishes the plant enzymes from their animal counterparts is their resistance to dideoxyribonucleotides.
从培养的大豆细胞的叶绿体和线粒体中纯化了 DNA 聚合酶。叶绿体酶有两种形式，它们在生化上无法区分。三种细胞器酶的分子量估计在 85000 到 90000 之间，并且在体外优先选择 poly(rA)dT(12-18)作为模板。叶绿体和线粒体 DNA 聚合酶的最大活性需要 KCl 和还原剂，并且这些酶对 DNA 聚合酶α的抑制剂完全有抗性。综上所述，这些特性将大豆细胞器酶归类为 DNA 聚合酶γ。植物酶与动物酶的一个独特区别是它们对双脱氧核苷酸的抗性。
