Krämer A, Haars R, Kabisch R, Will H, Bautz F A, Bautz E K
Mol Gen Genet. 1980;180(1):193-9. doi: 10.1007/BF00267369.
Monoclonal antibodies were raised against purified RNA polymerase II ( or B) from Drosophila melanogaster. The antibody produced by one hybridoma cell clone was found to be directed against the two large subunits of the enzyme. The absence of antibodies directed against proteins possibly contaminating the antigens used for immunization allowed us to identify RNA polymerase unequivocally in interbands and puffs of polytene chromosomes. Within a single heat shock puff (87C1) RNA polymerase was found to be clustered in two separate areas suggesting two distinct regions of RNA polymerase activity in this puff.
制备了针对果蝇纯化的RNA聚合酶II（或B）的单克隆抗体。发现一个杂交瘤细胞克隆产生的抗体针对该酶的两个大亚基。由于不存在针对可能污染用于免疫的抗原的蛋白质的抗体，使我们能够在多线染色体的间带和胀泡中明确鉴定RNA聚合酶。在单个热休克胀泡（87C1）内，发现RNA聚合酶聚集在两个分开的区域，表明该胀泡中有两个不同的RNA聚合酶活性区域。
