Abram J W, Nedwell D B
Arch Microbiol. 1978 Apr 27;117(1):89-92. doi: 10.1007/BF00689356.
A methanogenic bacterial consortium was obtained after inoculation of benzoate medium under N2/CO2 atmosphere with intertidal sediment. A hydrogen donating organotroph and Methanococcus mazei were isolated from this enrichment. H2-utilising sulphate reducing bacteria were isolated under H2/CO2 in the absence of organic electron donors. The Methanococcus was able to produce methane in yeast extract medium under N2/CO2 if the H2 donating organism was present, and sulphate reduction occurred if the hydrogen utilising sulphate reducing bacteria were grown with the H2 donating organism. The ability of the H2 utilising sulphate reducing bacteria to inhibit Methanococcus competitively was shown in clutures containing both of these H2 utilising bacteria.
在氮气/二氧化碳气氛下，将潮间带沉积物接种到苯甲酸盐培养基中后，获得了一个产甲烷细菌群落。从这种富集培养物中分离出了一种供氢有机营养菌和马氏甲烷球菌。在没有有机电子供体的情况下，在氢气/二氧化碳条件下分离出了利用氢气的硫酸盐还原菌。如果存在供氢生物体，马氏甲烷球菌能够在氮气/二氧化碳条件下的酵母提取物培养基中产生甲烷；如果利用氢气的硫酸盐还原菌与供氢生物体一起生长，则会发生硫酸盐还原。在含有这两种利用氢气的细菌的培养物中，显示出利用氢气的硫酸盐还原菌竞争性抑制马氏甲烷球菌的能力。
