通过对血液单核细胞 - 血小板部分进行培养，在采集标本后18小时内鉴定出伤寒杆菌，从而实现伤寒热的快速诊断。
Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood.
作者信息
Rubin F A, McWhirter P D, Burr D, Punjabi N H, Lane E, Kumala S, Sudarmono P, Pulungsih S P, Lesmana M, Tjaniadi P
机构信息
Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
出版信息
J Clin Microbiol. 1990 Apr;28(4):825-7. doi: 10.1128/jcm.28.4.825-827.1990.
Abstract
Detection of Salmonella typhi in blood by culture of the mononuclear cell-platelet layer was compared with other methods currently used for the diagnosis of typhoid fever. Colonies of S. typhi were present in all mononuclear cell-platelet layer-positive cultures within 18 h of plating and were identified within an additional 10 min by a coagglutination technique. In contrast, identification of all positive cultures by conventional blood culture required 3 days.
摘要
通过单核细胞 - 血小板层培养检测血液中伤寒沙门氏菌，并与目前用于诊断伤寒热的其他方法进行比较。接种后18小时内，所有单核细胞 - 血小板层阳性培养物中均出现伤寒沙门氏菌菌落，并在另外10分钟内通过协同凝集技术进行鉴定。相比之下，通过传统血培养鉴定所有阳性培养物需要3天。
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Detection of Salmonella C1, D and V1 antigens, by coagglutination, in blood cultures from patients with Salmonella infections.采用协同凝集法检测沙门氏菌感染患者血培养物中的沙门氏菌C1、D和V1抗原。
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Duodenal string-capsule culture compared with bone-marrow, blood, and rectal-swab cultures for diagnosing typhoid and paratyphoid fever.十二指肠线囊培养与骨髓、血液及直肠拭子培养用于诊断伤寒和副伤寒热的比较
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