Aubert C, Janiaud P, Rouge F, Hansson C, Rorsman H, Rosengren E
Ann Clin Res. 1980 Dec;12(6):288-94.
The catecholic amino acids, dopa and 5-S-cysteinyldopa, and the dopamine metabolite, homovanillic acid, were determined in 8 neuroblastomas. 5-S-Cysteinyldopa and/or dopa were detected in all cases and homovanillic acid was present in 5. Dopa was also found in two tumours in which no definite histological diagnosis could be made. Neuroblastoma cells were cultured from 7 patients. The ageing of human sympathoblasts in culture was accompanied by modifications in the ability to synthetize dopa, which is a precursor of catecholamines as well as of melanins, and the metabolite homovanillic acid. An increase in the levels of 5-S-cysteinyldopa, a metabolite of the melanocytes, has been observed. Concurrent modifications of ultrastructural morphology with the disappearance of granular vesicles and appearance of melanosomes were noticed. This modulation of the original phenotypic expression commonly resulted in cell death, but in one case of metastatic adenopathy of a neuroblastoma we have been able to establish a permanent pigmented cell line.
在8例神经母细胞瘤中测定了儿茶酚胺氨基酸、多巴和5-S-半胱氨酰多巴以及多巴胺代谢产物高香草酸。所有病例均检测到5-S-半胱氨酰多巴和/或多巴，5例中存在高香草酸。在两例无法做出明确组织学诊断的肿瘤中也发现了多巴。从7例患者中培养出神经母细胞瘤细胞。培养的人交感神经母细胞老化伴随着合成多巴（儿茶酚胺以及黑色素的前体）和代谢产物高香草酸能力的改变。观察到黑素细胞代谢产物5-S-半胱氨酰多巴水平升高。同时注意到超微结构形态的改变，颗粒小泡消失，黑素小体出现。这种原始表型表达的调节通常导致细胞死亡，但在1例神经母细胞瘤转移性腺病中，我们成功建立了一个永久性色素沉着细胞系。
