Hank J A, Chan J K, Edwards M L, Muller D, Smith D W
J Infect Dis. 1981 May;143(5):734-8. doi: 10.1093/infdis/143.5.734.
The protective efficacy of two bacille Calmette-Guérin (BCG) vaccines was examined in guinea pigs infected by the respiratory route with strains of Mycobacterium tuberculosis differing in virulence. Virulence was defined as the degree of tissue damage (weight) of primary lesions excised from lungs of unvaccinated guinea pigs killed 28-42 days after infection. Groups of animals vaccinated with BCG-Copenhagen (strain no. 1331), a vaccine of high potency, or those vaccinated with BCG-Prague (strain no. 725), an experimental vaccine of low potency, and groups given placebo were challenged six weeks later with one of three challenge strains differing in virulence. Protection was assessed from the difference in the number of tubercle bacilli recovered from excised primary lung lesions or from primary lesion-free lung lobes of vaccinated vs. unvaccinated animals. The virulence of the challenge strain influenced the efficacy of BCG vaccination; however, the results of other studies with a laboratory strain were in general replicated.
用毒力不同的结核分枝杆菌菌株经呼吸道感染豚鼠，以此检验两种卡介苗（BCG）的保护效力。毒力定义为感染后28 - 42天处死的未接种疫苗豚鼠肺中切除的原发性病变的组织损伤程度（重量）。分别用高效疫苗卡介苗 - 哥本哈根（菌株编号1331）、低效实验性疫苗卡介苗 - 布拉格（菌株编号725）对动物组进行接种，并设置给予安慰剂的组，六周后用三种毒力不同的攻击菌株之一对这些组进行攻击。通过比较接种疫苗和未接种疫苗动物切除的原发性肺部病变或无原发性病变的肺叶中回收的结核杆菌数量差异来评估保护效果。攻击菌株的毒力影响卡介苗接种的效力；然而，其他关于实验室菌株的研究结果总体上得到了重复。
