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来自与着丝粒β-异染色质相邻区域的黑腹果蝇基因的分子克隆及初步表征

Molecular cloning and preliminary characterization of a Drosophila melanogaster gene from a region adjacent to the centromeric beta-heterochromatin.

作者信息

Biessmann H, Kuger P, Schröpfer C, Spindler E

出版信息

Chromosoma. 1981;82(4):493-503. doi: 10.1007/BF00295008.

DOI:10.1007/BF00295008
PMID:6790248
Abstract

Using recombinant DNA technology we have isolated a 4.4 kb DNA fragment from Drosophila melanogaster which can be localized by in situ hybridization to the region 80C on the left arm of chromosome III. This DNA fragment codes for a 1.4 kb long poly(A)-containing RNA which comprises about 0.6% of the mass of cytoplasmic poly(A) RNA in Kc cells and Oregon R Embryos. This RNA codes for a 26,000 MW protein of still unknown function.

摘要

利用重组DNA技术,我们从黑腹果蝇中分离出一个4.4 kb的DNA片段,通过原位杂交可将其定位到三号染色体左臂的80C区域。这个DNA片段编码一种1.4 kb长的含poly(A)的RNA,它在Kc细胞和俄勒冈R胚胎的细胞质poly(A) RNA中约占0.6%。这种RNA编码一种功能仍未知的26,000 MW的蛋白质。

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1
Molecular cloning and preliminary characterization of a Drosophila melanogaster gene from a region adjacent to the centromeric beta-heterochromatin.来自与着丝粒β-异染色质相邻区域的黑腹果蝇基因的分子克隆及初步表征
Chromosoma. 1981;82(4):493-503. doi: 10.1007/BF00295008.
2
[Microcloning and characteristics of DNA from regions of the centromeric heterochromatin of Drosophila melanogaster polytene chromosomes].[黑腹果蝇多线染色体着丝粒异染色质区域DNA的微克隆及特性]
Genetika. 1999 Jan;35(1):55-61.
3
One of the copia genes is adjacent to satellite DNA in Drosophila melanogaster.在黑腹果蝇中,其中一个copia基因与卫星DNA相邻。
Cell. 1978 Nov;15(3):733-42. doi: 10.1016/0092-8674(78)90259-3.
4
Identification of Porto-1, a new repeated sequence that localises close to the centromere of chromosome 2 of Drosophila melanogaster.鉴定Porto-1,一种新的重复序列,其定位于黑腹果蝇2号染色体着丝粒附近。
Chromosoma. 1996 Oct;105(4):211-22.
5
A gene adjacent to satellite DNA in Drosophila melanogaster.果蝇中与卫星DNA相邻的一个基因。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5898-902. doi: 10.1073/pnas.75.12.5898.
6
Transcription of intercalary heterochromatin regions in Drosophila melanogaster cell culture.黑腹果蝇细胞培养中居间异染色质区域的转录
Chromosoma. 1980;80(2):177-90. doi: 10.1007/BF00286299.
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Dynamic organization of the beta-heterochromatin in the Drosophila melanogaster polytene X chromosome.果蝇多线X染色体中β-异染色质的动态组织
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A cloned unique gene of Drosophila melanogaster contains a repetitive 3' exon whose sequence is present at the 3' ends of many different mRNAs.一个克隆的黑腹果蝇独特基因包含一个重复的3'外显子,其序列存在于许多不同mRNA的3'末端。
Cell. 1982 Feb;28(2):365-73. doi: 10.1016/0092-8674(82)90354-3.
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[Drosophila beta-heterochromatin: molecular organization and function. Cloning and molecular biological analysis of the lambda 20 DNA fragment from Drosophila melanogaster beta-heterochromatin].[果蝇β-异染色质:分子组织与功能。黑腹果蝇β-异染色质λ20 DNA片段的克隆及分子生物学分析]
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[Transcription of portions of chromosomes corresponding to regions of intercalary heterochromatin in Drosophila melanogaster cell cultures].[果蝇细胞培养中与居间异染色质区域相对应的部分染色体的转录]
Genetika. 1980;16(10):1729-40.

引用本文的文献

1
Genetic Analysis of the Heterochromatin of Chromosome 3 in Drosophila Melanogaster. II. Vital Loci Identified through Ems Mutagenesis.果蝇染色体 3 异染色质的遗传分析。II. 通过 EMS 诱变鉴定的生活必需基因座位。
Genetics. 1988 Oct;120(2):519-32. doi: 10.1093/genetics/120.2.519.
2
The heterochromatic rolled gene of Drosophila melanogaster is extensively polytenized and transcriptionally active in the salivary gland chromocenter.果蝇的异染色质卷曲基因在唾液腺染色中心广泛多线化且具有转录活性。
Genetics. 1996 Sep;144(1):117-25. doi: 10.1093/genetics/144.1.117.
3
Identification and germline transformation of the ribosomal protein rp21 gene of Drosophila: complementation analysis with the Minute QIII locus reveals nonidentity.

本文引用的文献

1
Cytogenetic analysis of major heterochromatic elements (especially Xh and Y) in Drosophila melanogaster, and the theory of "heterochromatin".黑腹果蝇主要异染色质成分(尤其是Xh和Y)的细胞遗传学分析以及“异染色质”理论
Chromosoma. 1959;10:535-88. doi: 10.1007/BF00396588.
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The genetic analysis of D. melanogaster heterochromatin.黑腹果蝇异染色质的遗传分析。
Cell. 1980 Oct;21(3):607-19. doi: 10.1016/0092-8674(80)90424-9.
3
Cloned complementary deoxyribonucleic acid of Drosophila cells. Relationship of genome copy number to messenger ribonucleic acid abundance.
果蝇核糖体蛋白rp21基因的鉴定与种系转化:与微小QIII位点的互补分析显示不同一性。
Mol Gen Genet. 1988 Aug;213(2-3):354-8. doi: 10.1007/BF00339602.
4
The organization and expression of the light gene, a heterochromatic gene of Drosophila melanogaster.果蝇黑腹果蝇的一个异染色质基因——轻基因的组织与表达。
Genetics. 1990 May;125(1):129-40. doi: 10.1093/genetics/125.1.129.
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4
Concentrations of individual RNA sequences in polyadenylated nuclear and cytoplasmic RNA populations of Drosophila cells.果蝇细胞多聚腺苷酸化核RNA和细胞质RNA群体中单个RNA序列的浓度。
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.
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Transcription of intercalary heterochromatin regions in Drosophila melanogaster cell culture.黑腹果蝇细胞培养中居间异染色质区域的转录
Chromosoma. 1980;80(2):177-90. doi: 10.1007/BF00286299.
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Changes in the abundance of polyadenylated RNAs in development of Drosophila melanogaster analyzed with cloned DNA fragments.
Dev Biol. 1981 Apr 15;83(1):62-8. doi: 10.1016/s0012-1606(81)80008-5.
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A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
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Non replicating DNA in Drosophila.果蝇中的非复制性DNA。
Genetics. 1969;61(1):Suppl:227-38.
10
Reptitive DNA sequences in drosophila.果蝇中的重复DNA序列。
Chromosoma. 1971;33(3):319-44. doi: 10.1007/BF00284948.