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通过酶联免疫吸附测定法(ELISA)对淋病进行血清学诊断。

Serological diagnosis of gonorrhoea by an enzyme-linked immunosorbent assay (Elisa).

作者信息

Glynn A A, Ison C

出版信息

Br J Vener Dis. 1978 Apr;54(2):97-102. doi: 10.1136/sti.54.2.97.

DOI:10.1136/sti.54.2.97
PMID:416879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1046369/
Abstract

IgG antibody to an outer membrane protein extracted from Neisseria gonorrhoeae was measured in patients with gonorrhoea. The level in such patients was significantly higher than in normal controls or in patients with other conditions who were attending the clinic for sexually transmitted diseases. Significantly higher antibody levels were detectable in half the patients within a few days of infection and in a similar proportion of carriers--both male and female. Men with rectal gonorrhoea had particularly high antibody levels. Sixteen per cent of presumptively normal men and 11% of normal women gave positive results but the actual false positive rate could be lower.

摘要

对从淋病奈瑟菌中提取的一种外膜蛋白的IgG抗体在淋病患者中进行了检测。此类患者体内的抗体水平显著高于正常对照组或前来诊所就诊的其他性传播疾病患者。在感染后的几天内,半数患者以及相似比例的携带者(包括男性和女性)体内可检测到显著更高的抗体水平。患有直肠淋病的男性抗体水平尤其高。16%的推定正常男性和11%的正常女性检测结果呈阳性,但实际的假阳性率可能更低。

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Serological diagnosis of gonorrhoea by an enzyme-linked immunosorbent assay (Elisa).通过酶联免疫吸附测定法(ELISA)对淋病进行血清学诊断。
Br J Vener Dis. 1978 Apr;54(2):97-102. doi: 10.1136/sti.54.2.97.
2
Enzyme-linked immunosorbent assay for detecting gonococcal antibodies using two antigenically different gonococcal pili as antigen.使用两种抗原性不同的淋球菌菌毛作为抗原的酶联免疫吸附测定法检测淋球菌抗体。
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Serological diagnosis of gonorrhoea by enzyme linked immunosorbent assay.酶联免疫吸附测定法用于淋病的血清学诊断。
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Enzyme-linked immunosorbent assay (ELISA) to detect antibodies in gonorrhea using whole cells.使用全细胞的酶联免疫吸附测定(ELISA)检测淋病抗体。
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S Afr Med J. 1983 Sep 17;64(12):451-4.
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引用本文的文献

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J Clin Microbiol. 1982 Apr;15(4):668-76. doi: 10.1128/jcm.15.4.668-676.1982.
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Enzyme-linked immunosorbent assay (ELISA) to detect antibodies in gonorrhea using whole cells.使用全细胞的酶联免疫吸附测定(ELISA)检测淋病抗体。
J Clin Pathol. 1981 Sep;34(9):1040-3. doi: 10.1136/jcp.34.9.1040.
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Infect Immun. 1980 Apr;28(1):101-10. doi: 10.1128/iai.28.1.101-110.1980.
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Application of immuno-blotting to the differentiation of specific antibodies in gonorrhoea.免疫印迹法在淋病特异性抗体鉴别中的应用。
Immunology. 1984 Mar;51(3):615-21.
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Gonococcal serology. A comparison of three different tests.淋球菌血清学。三种不同检测方法的比较。
Br J Vener Dis. 1983 Feb;59(1):47-52. doi: 10.1136/sti.59.1.47.

本文引用的文献

1
Nature and Heterogeneity of the Antigens of Neisseria gonorrhoeae Involved in the Serum Bactericidal Reaction.淋病奈瑟菌血清杀菌反应相关抗原的性质和异质性。
Infect Immun. 1970 Aug;2(2):162-8. doi: 10.1128/iai.2.2.162-168.1970.
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A comparison of serological tests for the diagnosis of gonorrhoea.用于淋病诊断的血清学检测比较。
Br J Vener Dis. 1971 Dec;47(6):448-51. doi: 10.1136/sti.47.6.448.
3
Human antibody response to lipopolysaccharides from Neisseria gonorrhoeae.人类对淋病奈瑟菌脂多糖的抗体反应。
J Clin Pathol. 1972 Jan;25(1):56-9. doi: 10.1136/jcp.25.1.56.
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Quantitative determination of antibody to gonococcal pili. Changes in antibody levels with gonococcal infection.淋菌菌毛抗体的定量测定。淋菌感染时抗体水平的变化。
J Clin Invest. 1973 Nov;52(11):2896-909. doi: 10.1172/JCI107486.
5
Serum antibody response in experimental human gonorrhoea. Immunoglobulins G, A, and M.实验性人类淋病中的血清抗体反应。免疫球蛋白G、A和M。
Br J Vener Dis. 1969 Dec;45(4):325-7. doi: 10.1136/sti.45.4.325.
6
Human serum antibodies reacting with endotoxin from Neisseria gonorrhoeae.与淋病奈瑟菌内毒素发生反应的人血清抗体。
Br J Vener Dis. 1971 Aug;47(4):269-72. doi: 10.1136/sti.47.4.269.
7
Antigenic analysis of Neisseria gonorrhoeae by crossed immunoelectrophoresis.用交叉免疫电泳法对淋病奈瑟菌进行抗原分析。
Infect Immun. 1976 Apr;13(4):1273-88. doi: 10.1128/iai.13.4.1273-1288.1976.
8
Studies on lipopolysaccharides isolated from strains of Neisseria gonorrhoeae.从淋病奈瑟菌菌株中分离出的脂多糖的研究。
J Gen Microbiol. 1975 May;88(1):123-31. doi: 10.1099/00221287-88-1-123.
9
Asymptomatic females: detection of antibody activity to gonococcal pili antigen by radioimmunoassay.无症状女性:通过放射免疫测定法检测针对淋球菌菌毛抗原的抗体活性。
J Clin Microbiol. 1977 Jan;5(1):26-30. doi: 10.1128/jcm.5.1.26-30.1977.
10
The lipopolysaccharides of Neisseria gonorrhoeae colony types 1 and 4.
Can J Biochem. 1975 May;53(5):623-9. doi: 10.1139/o75-084.