Shilo B Z, Weinberg R A
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6789-92. doi: 10.1073/pnas.78.11.6789.
Sequences homologous to the oncogene sequences of acute RNA tumor viruses have been shown to be highly conserved within vertebrates. In the present work, eight different oncogene DNA sequences have been used as probes to search for homologous sequences in the DNA of organisms of other phyla. Five of these probes hybridized to the DNA of Drosophila melanogaster. Abelson leukemia virus probe detected a single homologous DNA fragment in Drosophila DNA. In contrast, probes prepared from the genomes of Harvey, avian, and feline sarcoma viruses and avian myelocytomatosis virus hybridized with multiple homologous sequences in Drosophila DNA. The identification of sequences homologous to vertebrate oncogenes in invertebrates demonstrates both a high degree of conservation of these genes and a wide distribution among divergent species. It seems likely that sequences homologous to vertebrate oncogenes play a crucial role in metazoan metabolism.
与急性RNA肿瘤病毒的致癌基因序列同源的序列已被证明在脊椎动物中高度保守。在本研究中，八个不同的致癌基因DNA序列被用作探针，以在其他门生物的DNA中寻找同源序列。其中五个探针与黑腹果蝇的DNA杂交。阿贝尔森白血病病毒探针在果蝇DNA中检测到一个单一的同源DNA片段。相比之下，从哈维、禽、猫肉瘤病毒和禽骨髓细胞瘤病毒基因组制备的探针与果蝇DNA中的多个同源序列杂交。在无脊椎动物中鉴定出与脊椎动物致癌基因同源的序列，既证明了这些基因的高度保守性，也证明了它们在不同物种中的广泛分布。与脊椎动物致癌基因同源的序列似乎在后生动物代谢中起关键作用。
