Høiby N, Hertz J B
Acta Pathol Microbiol Scand C. 1981 Jun;89(3):185-92. doi: 10.1111/j.1699-0463.1981.tb02684.x.
A quantitative determination of anti-Pseudomonas immunoglobulins was carried out by means of rocket-line immunoelectrophoresis in the serum from 19 cystic fibrosis (CF) patients with chronic P. aeruginosa infection and with many precipitins against these bacteria (CF + P). from six CF patients without P. aeruginosa infection (CF-P) and from nine normal persons. On an average P. aeruginosa antigens could absorb 7.7% of IgG, 8.4% of IgA and 29% of IgM from CF + P sera, whereas no detectable IgG and IgA and only 14.6% IgM were absorbed from normal sera and only 1.2% of IgG, 3.8% of IgA, but 29% of IgM was absorbed from CF-P sera. The results show that most, if not all, of the P. aeruginosa precipitins belong to the IgG and IgA classes, but that these precipitins can account only for part of the increased levels of immunoglobulins in CF-P patients. Staphylococcus aureus containing protein A (strain Cowan 1) could absorb 95% of the precipitating antibodies against P. aeruginosa and 92% of IgG, 27% of IgA and 34% of IgM in CF-P patients. The absorption of P. aeruginosa precipitins by protein A points to a possible synergism between S. aureus and P. aeruginosa infections in the lungs of CF patients, since S. aureus may interfere with antibody-mediated immune elimination of P. aeruginosa. Such a mechanism may also facilitate infections with other microorganisms in these patients.
采用火箭免疫电泳法对19例患有慢性铜绿假单胞菌感染且对这些细菌有多种沉淀素的囊性纤维化（CF）患者（CF + P）、6例无铜绿假单胞菌感染的CF患者（CF - P）以及9名正常人血清中的抗铜绿假单胞菌免疫球蛋白进行了定量测定。平均而言，铜绿假单胞菌抗原可从CF + P血清中吸收7.7%的IgG、8.4%的IgA和29%的IgM，而从正常血清中未检测到可吸收的IgG和IgA，仅吸收14.6%的IgM，从CF - P血清中仅吸收1.2%的IgG、3.8%的IgA，但吸收29%的IgM。结果表明，大多数（如果不是全部）铜绿假单胞菌沉淀素属于IgG和IgA类别，但这些沉淀素只能解释CF - P患者免疫球蛋白水平升高的一部分。含有蛋白A的金黄色葡萄球菌（考恩1菌株）可吸收CF - P患者中95%的抗铜绿假单胞菌沉淀抗体以及92%的IgG、27%的IgA和34%的IgM。蛋白A对铜绿假单胞菌沉淀素的吸收表明，CF患者肺部金黄色葡萄球菌和铜绿假单胞菌感染之间可能存在协同作用，因为金黄色葡萄球菌可能会干扰抗体介导的对铜绿假单胞菌的免疫清除。这种机制也可能会促进这些患者感染其他微生物。
