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6-cyanopurine, a color indicator useful for isolating mutations in the nif (nitrogen fixation) genes of Klebsiella pneumoniae.6-氰基嘌呤，一种用于分离肺炎克雷伯菌固氮（nif）基因中突变的颜色指示剂。
J Bacteriol. 1978 Oct;136(1):247-52. doi: 10.1128/jb.136.1.247-252.1978.
2
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4
Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.在铵存在的情况下，导致肺炎克雷伯菌对固氮酶合成去阻遏的固氮基因中的突变。
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Temperature sensitivity of the regulation of nitrogenase synthesis by Klebsiella pneumoniae.肺炎克雷伯菌对固氮酶合成调控的温度敏感性
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4
Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.在铵存在的情况下，导致肺炎克雷伯菌对固氮酶合成去阻遏的固氮基因中的突变。
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7
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8
Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.肺炎克雷伯菌中固氮基因（nif）的精细结构图谱绘制与互补分析
J Bacteriol. 1978 Oct;136(1):253-66. doi: 10.1128/jb.136.1.253-266.1978.

本文引用的文献

1
BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI.噬菌体诱导的大肠杆菌突变
Proc Natl Acad Sci U S A. 1963 Dec;50(6):1043-51. doi: 10.1073/pnas.50.6.1043.
2
O0 and strong-polar mutations in the gal operon are insertions.O0和gal操纵子中的强极性突变是插入突变。
Mol Gen Genet. 1968;102(4):353-63. doi: 10.1007/BF00433726.
3
Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae.肺炎克雷伯菌中固氮基因的转导
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174-7. doi: 10.1073/pnas.68.6.1174.
4
A comparison of the specificities of xanthine oxidase and aldehyde oxidase.黄嘌呤氧化酶与醛氧化酶特异性的比较。
Arch Biochem Biophys. 1972 Jun;150(2):585-99. doi: 10.1016/0003-9861(72)90078-1.
5
Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae.肺炎克雷伯菌中通过接合作用转移固氮基因。
Nature. 1971 Nov 5;234(5323):47-8. doi: 10.1038/234047a0.
6
On the origin and direction of replication of the Escherichia coli K12 chromosome.关于大肠杆菌K12染色体复制的起始点和方向
J Mol Biol. 1968 Mar 28;32(3):611-29. doi: 10.1016/0022-2836(68)90346-x.
7
Effect of molybdenum starvation and tungsten on the synthesis of nitrogenase components in Klebsiella pneumonia.钼饥饿和钨对肺炎克雷伯菌中固氮酶组分合成的影响。
J Bacteriol. 1974 Jun;118(3):986-9. doi: 10.1128/jb.118.3.986-989.1974.
8
An improved bacterial test system for the detection and classification of mutagens and carcinogens.一种用于检测诱变剂和致癌物并对其进行分类的改良细菌检测系统。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):782-6. doi: 10.1073/pnas.70.3.782.
9
Prophage deletion mapping of bacteriophage Mu-1.噬菌体Mu-1的原噬菌体缺失图谱分析
Virology. 1973 Jul;54(1):93-101. doi: 10.1016/0042-6822(73)90118-9.
10
Studies of the binding of adenine to adenine phosphoribosyltransferase.腺嘌呤与腺嘌呤磷酸核糖转移酶结合的研究。
Can J Biochem. 1970 Mar;48(3):295-301. doi: 10.1139/o70-051.

6-氰基嘌呤，一种用于分离肺炎克雷伯菌固氮（nif）基因中突变的颜色指示剂。

6-cyanopurine, a color indicator useful for isolating mutations in the nif (nitrogen fixation) genes of Klebsiella pneumoniae.

作者信息

MacNeil D, Brill W J

出版信息

J Bacteriol. 1978 Oct;136(1):247-52. doi: 10.1128/jb.136.1.247-252.1978.

DOI:10.1128/jb.136.1.247-252.1978
PMID:361692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC218655/
Abstract

6-Cyanopurine (6-CP) can be used as a color indicator for certain classes of nif (N2 fixation) mutations in Klebsiella pneumoniae. Under N2-fixing conditions, Nif+ colonies and most Nif- colonies are purple on media containing 6-CP. Twenty-two Nif- mutants with altered color on medium containing 6-CP were isolated. All white mutants contained mutations in the regulatory genes, nifAA-nifL. Mutants which were more darkly colored than the wild type had mutations distributed among six nif genes. Medium with 6-CP was used to isolate Nif- mutants with deletions internal to the nif genes, and 6-CP was used to identify strains depressed for nitrogenase synthesis in the presence of NH4+.

摘要

6-氰基嘌呤（6-CP）可作为肺炎克雷伯菌中某些类型固氮（nif，即N₂固定）突变的颜色指示剂。在固氮条件下，Nif⁺菌落和大多数Nif⁻菌落在含有6-CP的培养基上呈紫色。分离出了22个在含有6-CP的培养基上颜色发生改变的Nif⁻突变体。所有白色突变体在调控基因nifAA-nifL中含有突变。颜色比野生型更深的突变体的突变分布在6个nif基因中。含有6-CP的培养基用于分离nif基因内部有缺失的Nif⁻突变体，并且6-CP用于鉴定在存在NH₄⁺时固氮酶合成受抑制的菌株。