Hayes J, Chappel R J
J Hyg (Lond). 1982 Feb;88(1):21-8. doi: 10.1017/s0022172400069850.
The serology of 27 heifers found to be positive to culture after inoculation with Brucella abortus strain 544, was studied. Eighteen heifers had previously been vaccinated with strain 19 or strain 45/20 and nine were unvaccinated. Post-infection serum samples were tested for Brucella antibodies by radioimmunoassay (RIA), complement fixation test (CFT), indirect haemolysis test (IHLT) and Rose Bengal plate test (RBPT). All of the unvaccinated heifers showed strong humoral responses to experimental infection in the RIA, CFT, IHLT and RBPT. The CFT and RBPT became positive sooner after infection than the other tests in the unvaccinated heifers. However, in vaccinated heifers the RIA was the most sensitive test early in infection and the results of the RBPT were variable. Three of the vaccinated heifers showed weak and inconsistent humoral responses and, in these animals, the RIA gave fewer false negative reactions than the other tests.
对27头接种流产布鲁氏菌544株后培养呈阳性的小母牛的血清学进行了研究。18头小母牛先前已接种19株或45/20株疫苗，9头未接种。通过放射免疫测定法（RIA）、补体结合试验（CFT）、间接溶血试验（IHLT）和玫瑰红平板试验（RBPT）对感染后血清样本进行布鲁氏菌抗体检测。所有未接种疫苗的小母牛在RIA、CFT、IHLT和RBPT中均显示出对实验性感染的强烈体液反应。在未接种疫苗的小母牛中，CFT和RBPT在感染后比其他检测更早呈阳性。然而，在接种疫苗的小母牛中，RIA在感染早期是最敏感的检测方法，而RBPT的结果则各不相同。3头接种疫苗的小母牛显示出微弱且不一致的体液反应，在这些动物中，RIA产生的假阴性反应比其他检测少。
