Saiki O, Kishimoto T, Kuritani T, Muraguchi A, Yamamura Y
J Immunol. 1980 Jun;124(6):2609-14.
In vitro stimulation of the B leukemic cells (B-CLL cells) with normal allogeneic T cells plus PWM induced IgM secretion and a switching from IgM to IgG production. Induction of IgM and IgG production in B-CLL cells with T cells was demonstrated by the presence of the same idiotype in induced Ig as that present in the monoclonal IgM protein in the patient's serum. Both T cells and PWM were required for Ig induction in B-CLL cells, and x-irradiated T cells showed the comparable helper effect. T cells and PWM induced not only Ig secretion but proliferation of B-CLL cells. Cell division was essential for the differentiation of the leukemic cells to Ig-producing cells. PWM-induced, antigen-nonspecific helper factor(s) were also effective in the induction of differentiation of the leukemic cells. Variations existed among T cell donors in the capabilities to induce differentiation of the same leukemic cells, suggesting the requirement of matching of acceptors on B-CLL cells and T effector molecules for the induction of Ig production in B-CLL cells.
用正常同种异体T细胞加PWM对B白血病细胞（B - CLL细胞）进行体外刺激，可诱导IgM分泌，并使产生的抗体从IgM转换为IgG。通过诱导产生的Ig中存在与患者血清中单克隆IgM蛋白相同的独特型，证明了T细胞可诱导B - CLL细胞产生IgM和IgG。B - CLL细胞中Ig的诱导需要T细胞和PWM，经X射线照射的T细胞显示出类似的辅助作用。T细胞和PWM不仅诱导Ig分泌，还诱导B - CLL细胞增殖。细胞分裂对于白血病细胞分化为产生Ig的细胞至关重要。PWM诱导的抗原非特异性辅助因子在白血病细胞分化诱导中也有效。不同T细胞供体诱导同一白血病细胞分化的能力存在差异，这表明B - CLL细胞上受体与T效应分子相匹配对于B - CLL细胞中Ig产生的诱导是必要的。
