亚急性硬化性全脑炎患者血清中的轻链谱
Light-chain profiles in sera from patients with subacute sclerosing panencephalitis.
作者信息
Mehta P D, Thormar H
出版信息
Immunol Lett. 1982 Feb;4(2):75-8. doi: 10.1016/0165-2478(82)90002-5.
Sera from 2 subacute sclerosing panencephalitis (SSPE) patients were absorbed with a concentrated preparation of measles virus. Measles-specific IgG was eluted from the precipitates containing measles antigen-antibody complex. These IgGs, when subjected to immunofixation after isoelectric focusing showed a number of oligoclonal bands with one type of light-(L) chain. In urea polyacrylamide gel electrophoresis, the reduced and alkylated measles-specific IgG showed 1-3 homogeneous L-chain bands, whereas IgG isolated from unabsorbed sera and IgG isolated from supernatants of SSPE sera after absorption with measles virus showed a diffuse L-chain band. It can be concluded that in SSPE, measles virus is responsible for the synthesis of L-chain with restricted heterogeneity.
从2例亚急性硬化性全脑炎（SSPE）患者的血清中用浓缩的麻疹病毒制剂进行吸收处理。从含有麻疹抗原-抗体复合物的沉淀物中洗脱麻疹特异性IgG。这些IgG在等电聚焦后进行免疫固定时，显示出多条寡克隆带，且只有一种轻链（L链）类型。在尿素聚丙烯酰胺凝胶电泳中，还原和烷基化后的麻疹特异性IgG显示出1 - 3条均一的L链带，而从未吸收血清中分离出的IgG以及用麻疹病毒吸收后从SSPE血清上清液中分离出的IgG显示出弥散的L链带。可以得出结论，在SSPE中，麻疹病毒导致了具有有限异质性的L链的合成。
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