Mehta P D, Patrick B A, Thormar H, Wisniewski H M
J Immunol. 1982 Nov;129(5):1983-5.
Oligoclonal IgG bands from SSPE sera were isolated by combination of Protein A-Sepharose 4B column and preparative isoelectric focusing gel procedures. Each eluted fraction, when examined in analytic IEF, showed two or three individual bands with isoelectric points close to one another, compared to approximately fifteen IgG bands seen in whole serum. When the bands were tested for measles antibody activity in immunofixation with measles virus followed by peroxidase staining, the bands eluted in pH region 8.5 to 9.3 were found to be measles specific, whereas those in pH 7.0 to 8.4 lacked significant measles activity. When eluted fractions containing groups of bands were absorbed with measles virus, the bands in pH region 8.5 to 9.3 were removed, whereas those in pH 7.0 to 8.4 region remained unchanged; this indicated that a number of oligoclonal IgG bands without measles virus activities are present in SSPE. The bands lacking measles-specific activity may be synthesized against other infectious agents or they may represent nonspecific activation of B cell clones.
通过蛋白A-琼脂糖4B柱和制备性等电聚焦凝胶程序相结合的方法，从亚急性硬化性全脑炎（SSPE）血清中分离出寡克隆IgG条带。在分析性等电聚焦中检测时，与全血清中大约15条IgG条带相比，每个洗脱组分显示出两到三条等电点彼此接近的单个条带。当用麻疹病毒进行免疫固定，随后进行过氧化物酶染色，检测这些条带的麻疹抗体活性时，发现在pH 8.5至9.3区域洗脱的条带具有麻疹特异性，而在pH 7.0至8.4区域的条带缺乏显著的麻疹活性。当用麻疹病毒吸收含有条带组的洗脱组分时，pH 8.5至9.3区域的条带被去除，而pH 7.0至8.4区域的条带保持不变；这表明在亚急性硬化性全脑炎血清中存在一些没有麻疹病毒活性的寡克隆IgG条带。缺乏麻疹特异性活性的条带可能是针对其他传染原合成的，或者它们可能代表B细胞克隆的非特异性激活。
