Lange C S, Steele V E
Differentiation. 1978 Jul 24;11(1):1-12. doi: 10.1111/j.1432-0436.1978.tb00965.x.
The substance which inhibits brain formation in the regenerating planarian Dugesia etrusca was found to be a large molecule, at least in part protein, which electrophoreses as an electronegative moiety in pH 6.8 buffer. A model is presented, based on this finding and previous studies, which proposes an electrochemical mechanism for the control of polarity and possibly for the maintenance of tissue organization in planarians. It is proposed that a bioelectric field exists and moves the electronegative brain-inhibiting substance in a posterior direction, establishing polarity. This model explains the polarity reversal experiments using external fields and many of the previously unexplained classical planarian experiments. Data are presented demonstrating the existence, magnitude, and polarity of this bioelectric field, which is not greatly altered upon decapitation, all in accord with predictions of the model.
研究发现，抑制再生涡虫杜氏真涡虫脑形成的物质是一种大分子，至少部分为蛋白质，在pH 6.8缓冲液中作为带负电的部分进行电泳。基于这一发现和先前的研究，提出了一个模型，该模型提出了一种电化学机制，用于控制涡虫的极性，并可能维持其组织的有序性。有人提出，生物电场存在并使带负电的脑抑制物质向后移动，从而确立极性。该模型解释了使用外部电场进行的极性反转实验以及许多以前无法解释的经典涡虫实验。给出的数据证明了这种生物电场的存在、大小和极性，断头后其变化不大，所有这些都与模型的预测一致。
