流产布鲁氏菌组分从血清中生成粒细胞和单核细胞趋化因子。
Generation of chemotactic factor for granulocytes and monocytes from serum by fractions of Brucella abortus.
作者信息
Birmingham J R, Tabatabai L B, Deyoe B L, Jeska E L, Nuessen M E
出版信息
Immunology. 1982 May;46(1):17-22.
Several fractions isolated from Brucella abortus were examined for their ability to generate chemotactic factor from normal serum. Phenol phase lipopolysaccharides exhibited activity equivalent to that obtained with E. Coli lipopolysaccharide. A carbohydrate-rich aqueous methanol fraction was inhibitory at high concentrations, but a non-dialysable component of this fraction contained a potent stimulator of chemotactic activity. Protein-rich fractions from both strain 19 and strain 2308 were inactive. Preheating the serum at 56 degrees for 30 min prevented generation of chemotactic activity by the various fractions, suggesting a role for serum complement. No chemotactic activity was produced by Brucella fractions in C5-deficient DBA/2J mouse serum.
对从流产布鲁氏菌中分离出的几个组分进行了检测，以考察它们从正常血清中产生趋化因子的能力。酚相脂多糖表现出与大肠杆菌脂多糖相当的活性。富含碳水化合物的甲醇水相组分在高浓度时具有抑制作用，但该组分的一个不可透析成分含有一种强大的趋化活性刺激物。来自19菌株和2308菌株的富含蛋白质的组分均无活性。将血清在56℃预热30分钟可阻止各种组分产生趋化活性，这表明血清补体发挥了作用。布鲁氏菌组分在C5缺陷的DBA/2J小鼠血清中不产生趋化活性。
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