小鼠λ轻链免疫球蛋白基因连接区的DNA序列。
DNA sequences of the joining regions of mouse lambda light chain immunoglobulin genes.
作者信息
Blomberg B, Tonegawa S
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(2):530-3. doi: 10.1073/pnas.79.2.530.
Abstract
The joining (J) segments of mouse immunoglobulin lambda light chain genes, lambda 2, lambda 3, and a presumptive lambda 4, were cloned, and their sequences were determined and compared with that of lambda 1. Although all the lambda J segments share sequence homology, the J1 and J4 segments and the J2 and J3 segments, respectively, are more homologous. These sequence data, together with the fact that present day lambda genes occur in two clusters, 5' J3C3J1C1 3' and 5' J2C2J4C4 3', further substantiates a probable evolutionary duplication unit, JIICIIJICI, with II the precursor of lambda 3 and lambda 2 and I the precursor of lambda I and lambda 4. From the J4 sequence, we conclude that the lambda 4 gene is most likely nonfunctional (i.e., a pseudogene). The signal nonamer sequence 5' to J3 differs from that of J1 in two consecutive base pairs. This difference could account in part for the lower level of expression of lambda 3 as compared with lambda 1 in mouse serum.
摘要
小鼠免疫球蛋白λ轻链基因的连接（J）片段，即λ2、λ3以及推测的λ4，被克隆出来，测定了它们的序列，并与λ1的序列进行比较。尽管所有的λJ片段都有序列同源性，但J1和J4片段以及J2和J3片段分别具有更高的同源性。这些序列数据，再加上当今的λ基因以两个簇的形式存在，即5' J3C3J1C1 3'和5' J2C2J4C4 3'，进一步证实了一个可能的进化重复单元JIICIIJICI，其中II是λ3和λ2的前体，而I是λI和λ4的前体。从J4序列我们得出结论，λ4基因很可能无功能（即假基因）。J3上游的信号九聚体序列在两个连续碱基对上与J1的不同。这种差异可能部分解释了与小鼠血清中的λ1相比，λ3表达水平较低的原因。
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本文引用的文献
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Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9. doi: 10.1073/pnas.78.6.3765.
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Immunoglobulin V/J recombination is accompanied by deletion of joining site and variable region segments.免疫球蛋白V/J重组伴随着连接位点和可变区片段的缺失。
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