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关于染色质间颗粒聚集体中染色质结构存在情况的高分辨率放射自显影研究。

High-resolution autoradiographic study on the presence of chromatin structures within interchromatin granule conglomerations.

作者信息

Gruca S, Krzyzowska-Gruca S, Zborek A

出版信息

Acta Histochem. 1981;69(2):255-63. doi: 10.1016/S0065-1281(81)80037-2.

DOI:10.1016/S0065-1281(81)80037-2
PMID:6805225
Abstract

In Ehrlich ascites tumour cell nuclei ledakrin (1-nitro-9/3'-dimethylpropyloamine/acridine-2 HCl) induces condensation and margination of chromatin, compaction and segregation of nucleolar components and conglomeration of chromatin granule clusters. Localization of DNA in nuclei of the cells labelled with 3H-thymidine and subsequently treated with ledakrin is studied using high-resolution autoradiography. Besides strong labelling of condensed chromatin some radioactivity is associated with IG conglomerations. Possible role of chromatin fibrils in the formation of conglomerations of IG is discussed.

摘要

在艾氏腹水肿瘤细胞核中,来达克林(1-硝基-9/3'-二甲基丙胺/吖啶-2盐酸盐)可诱导染色质浓缩和边缘化、核仁成分致密化和分离以及染色质颗粒簇聚集。使用高分辨率放射自显影术研究了用3H-胸腺嘧啶核苷标记并随后用 来达克林处理的细胞的细胞核中DNA的定位。除了浓缩染色质的强标记外,一些放射性与IG聚集体相关。讨论了染色质纤维在IG聚集体形成中的可能作用。

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引用本文的文献

1
Distribution of interchromatin granules in nuclear matrices obtained from nuclei exhibiting different degree of chromatin condensation.从表现出不同程度染色质凝聚的细胞核中获得的核基质中染色质间颗粒的分布。
Cell Tissue Res. 1983;231(2):427-37. doi: 10.1007/BF00222192.