Binz H, Meier B, Wigzell H
Int J Cancer. 1982 Apr 15;29(4):417-23. doi: 10.1002/ijc.2910290410.
DA rat sarcoma P1 and P2 were induced by dimethylbenz(a)anthracene. A tumor-specific immune response of DA rats against P1-tumor cells could be demonstrated at the humoral and cellular level. DA anti-P1 antibodies were purified on fixed P1-tumor cells and used as auto-immunogen in DA rats for the production of anti-idiotypic antibodies. Such anti-idiotypic antibodies could be demonstrated by using a solid-phase radioimmunoassay and by their ability to induce secondary type of DA anti-P1 response in vitro. In addition, such antibodies were able to induce cytotoxic T lymphocytes capable of eliminating P1-tumor cells but not control tumor cells. In some of the auto-immunized DA rats enhanced P1-tumor growth could be observed, indicating that the anti-idiotypic immune response had led to a selective ablation of idiotypic, potential anti-P1 reactive T cells.
二甲基苯并(a)蒽诱导产生了DA大鼠肉瘤P1和P2。在体液和细胞水平上均可证明DA大鼠针对P1肿瘤细胞存在肿瘤特异性免疫反应。DA抗P1抗体在固定的P1肿瘤细胞上进行纯化，并用作DA大鼠的自身免疫原以产生抗独特型抗体。通过固相放射免疫测定法以及它们在体外诱导DA抗P1反应二级类型的能力可以证明此类抗独特型抗体的存在。此外，此类抗体能够诱导细胞毒性T淋巴细胞，这些细胞毒性T淋巴细胞能够消除P1肿瘤细胞，但不能消除对照肿瘤细胞。在一些自身免疫的DA大鼠中，可以观察到P1肿瘤生长增强，这表明抗独特型免疫反应导致了独特型的、潜在的抗P1反应性T细胞的选择性消融。
