Selepak S T, Witebsky F G
J Clin Microbiol. 1985 Nov;22(5):835-7. doi: 10.1128/jcm.22.5.835-837.1985.
For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be required. S. aureus does not multiply in coagulase plasma. There is also lot-to-lot variation in the sensitivity of coagulase plasma. On isolates from critical specimens, a negative coagulase test obtained with an inoculum of less than two colonies should be confirmed by repeating the procedure with a larger inoculum or by using some other confirmatory test.
对于某些金黄色葡萄球菌分离株进行凝固酶阳性试验时，可能需要用至少两个菌落接种0.5 ml凝固酶血浆，并在35至37摄氏度下孵育24小时。金黄色葡萄球菌不会在凝固酶血浆中繁殖。凝固酶血浆的敏感性也存在批次间差异。对于来自关键标本的分离株，接种少于两个菌落得到的凝固酶试验阴性结果，应通过用更大接种量重复该程序或使用其他一些确证试验来确认。
