Pourshadi M, Klaas J
Diagn Microbiol Infect Dis. 1984 Sep;2(4):287-91. doi: 10.1016/0732-8893(84)90059-2.
In a blind study, a latex agglutination test (Serostat Staphylococcus, Scott Laboratories) and a microtube coagulase test (Staphase, API) were evaluated for their ability to detect Staphylococcus aureus. Of 289 isolates of catalase-positive, gram-positive cocci, 122 were identified as S. aureus based on positive reactions in at least three of the following tests: tube coagulase, slide coagulase, DNase production, or anaerobic fermentation of mannitol. The latex agglutination test gave positive reactions for all S. aureus isolates and 10 (6%) non-S. aureus isolates. The slide coagulase test was positive for 121 S. aureus isolates and three (2%) non-S. aureus isolates. The microtube coagulase test detected 53, 90, and 98% of the S. aureus isolates after 2, 4, and 24 hr, respectively. In contrast, the conventional tube coagulase test detected 97% of the S. aureus isolates after 2 hr, and 98% after 4 and 24 hr. Two isolates of S. aureus gave negative tube coagulase reactions at 37 degrees C, but positive reactions at room temperature after 24 hr. The combination of tube and slide coagulase tests provided the most reliable results. The slide and tube coagulase tests gave more reliable results than the latex agglutination and microtube coagulase tests, respectively.
在一项盲法研究中，对乳胶凝集试验（Serostat葡萄球菌，斯科特实验室）和微量管凝固酶试验（Staphase，API）检测金黄色葡萄球菌的能力进行了评估。在289株过氧化氢酶阳性的革兰氏阳性球菌分离株中，基于以下至少三项试验呈阳性反应，122株被鉴定为金黄色葡萄球菌：试管凝固酶试验、玻片凝固酶试验、DNA酶产生试验或甘露醇厌氧发酵试验。乳胶凝集试验对所有金黄色葡萄球菌分离株及10株（6%）非金黄色葡萄球菌分离株呈阳性反应。玻片凝固酶试验对121株金黄色葡萄球菌分离株及3株（2%）非金黄色葡萄球菌分离株呈阳性反应。微量管凝固酶试验在2、4和24小时后分别检测到53%、90%和98%的金黄色葡萄球菌分离株。相比之下，传统的试管凝固酶试验在2小时后检测到了97%的金黄色葡萄球菌分离株，在4小时和24小时后检测到98%。两株金黄色葡萄球菌分离株在37℃时试管凝固酶试验呈阴性反应，但在室温下24小时后呈阳性反应。试管和玻片凝固酶试验相结合提供了最可靠的结果。玻片和试管凝固酶试验分别比乳胶凝集试验和微量管凝固酶试验给出了更可靠的结果。
