Auckenthaler R, Ilstrup D M, Washington J A
J Clin Microbiol. 1982 May;15(5):860-4. doi: 10.1128/jcm.15.5.860-864.1982.
We compared blood cultures that were diluted 1:5 (20%, vol/vol) and 1:10 (10%, vol/vol) and contained specimens from patients with suspected septicemia. Streptococcus pneumoniae was recovered significantly more frequently from blood cultures diluted 20%, whereas gram-negative bacilli, group D streptococci, Staphylococcus aureus, and Candida spp. were recovered significantly sooner and more frequently from blood cultures diluted 10%. Statistically significant differences in isolation rates, however, represented only a small number of patients for whom the positive cultures affected therapy. We conclude that as long as at least two separate sets of blood cultures are obtained per septic episode from each patient, a 1:5 to 1:10 blood/vented (aerobic) medium ratio provides acceptable results. Nevertheless, the results also demonstrate that blood cultures diluted 10% provided greater and faster yields than those provided by blood cultures diluted 20%.
我们比较了稀释比例为1:5（20%，体积/体积）和1:10（10%，体积/体积）且包含疑似败血症患者标本的血培养物。肺炎链球菌在稀释20%的血培养物中检出频率显著更高，而革兰氏阴性杆菌、D组链球菌、金黄色葡萄球菌和念珠菌属在稀释10%的血培养物中检出时间显著更早且频率更高。然而，分离率的统计学显著差异仅代表少数阳性培养物影响治疗的患者。我们得出结论，只要每位患者每次败血症发作至少采集两组独立的血培养物，1:5至1:10的血液/通气（需氧）培养基比例可提供可接受的结果。尽管如此，结果也表明，稀释10%的血培养物比稀释20%的血培养物产生的产量更高且更快。
