Tomiwa K, Hazama F, Mikawa H
Acta Pathol Jpn. 1982 May;32(3):427-35. doi: 10.1111/j.1440-1827.1982.tb01399.x.
Histological and fluoromicroscopical studies were performed in order to obtain information about reversibility and associated tissue damage of an osmotic opening of the blood-brain barrier. Three ml of 1.4 M mannitol solution were perfused through the right carotid artery of a rat, and the opening of the blood-brain barrier was examined by using EVans blue as a tracer. The barrier was opened for 60-120 minutes and then reestablished without gross neurological defect. Microscopically, however, edematous change and microinfarcts were often observed, which might be due to microembolism of recrystallized mannitol. With filtration of the perfusate through a millipore filter, the blood-brain barrier was reversibly opened without any tissue damages. This could be a useful therapeutic technique and an experimental model for neurotoxicology.
为了获取有关血脑屏障渗透性开放的可逆性及相关组织损伤的信息，进行了组织学和荧光显微镜研究。向大鼠的右颈动脉灌注3毫升1.4 M甘露醇溶液，并使用伊文思蓝作为示踪剂检查血脑屏障的开放情况。血脑屏障开放60 - 120分钟，然后恢复，未出现明显的神经功能缺损。然而，在显微镜下，经常观察到水肿变化和微梗死，这可能是由于再结晶甘露醇的微栓塞所致。通过微孔滤器过滤灌注液，血脑屏障可逆性开放且无任何组织损伤。这可能是一种有用的治疗技术和神经毒理学的实验模型。
