Bygdeman S, Bäckman M, Danielsson D, Norgren M
Acta Pathol Microbiol Immunol Scand B. 1982 Jun;90(3):243-50. doi: 10.1111/j.1699-0463.1982.tb00112.x.
In previous works statistically significant differences were demonstrated in antibiotic susceptibility between gonococcal strains of the recently described W serogroups, W I, W II and W III, respectively. Strains of serogroup W I were almost always sensitive to penicillin and other antibiotics while those of the W II and W III serogroups showed a higher incidence of decreased susceptibility. Transformation experiments were therefore undertaken with an antibiotic sensitive serogroup W I gonococcal strain as recipient and a multi-resistant W II strain as DNA-donor. Transformants, with increased resistance to penicillin and several other antibiotics as compared with the recipient, acquired the same serogroup specificity as the W II donor. With one of these W II transformants as donor and the sensitive W I strain as recipient all transformants acquired the same antibiotic susceptibility pattern as well as the same serogroup as the donor. SDS-PAGE, performed on sarkosyl extracted outer membrane proteins from donor, recipient and some transformants, showed an increase in the molecular weight of the Protein I of the outer membrane of the W II transformants as compared with that of the recipient strain. In rocket-line and crossed-line immunoelectrophoresis the W II transformants could not be distinguished from the W II donor strain. A genetic linkage between antibiotic multi-resistance and serogroup W II specificity was thus shown. This is in agreement with the demonstrated higher incidence of W II strains with increased antibiotic resistance as compared with that of serogroup W I strains.
在先前的研究中，分别在最近描述的W血清群（W I、W II和W III）的淋球菌菌株之间的抗生素敏感性方面显示出统计学上的显著差异。W I血清群的菌株几乎总是对青霉素和其他抗生素敏感，而W II和W III血清群的菌株显示出更高的敏感性降低发生率。因此，进行了转化实验，以抗生素敏感的W I血清群淋球菌菌株作为受体，以多重耐药的W II菌株作为DNA供体。与受体相比，对青霉素和其他几种抗生素耐药性增加的转化体获得了与W II供体相同的血清群特异性。以这些W II转化体之一作为供体，以敏感的W I菌株作为受体，所有转化体都获得了与供体相同的抗生素敏感性模式以及相同的血清群。对来自供体、受体和一些转化体的十二烷基肌氨酸钠提取的外膜蛋白进行SDS-PAGE分析，结果显示，与受体菌株相比，W II转化体外膜的蛋白I分子量增加。在火箭免疫电泳和交叉免疫电泳中，无法区分W II转化体和W II供体菌株。因此，显示出抗生素多重耐药性与W II血清群特异性之间存在遗传联系。这与所证明的W II菌株抗生素耐药性增加的发生率高于W I血清群菌株的情况一致。
