与小鼠胞质A颗粒相关的一种RNA指导的DNA聚合酶的特性分析。

Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles.

作者信息

Kohno M, Tanaka H

出版信息

J Virol. 1977 May;22(2):273-80. doi: 10.1128/JVI.22.2.273-280.1977.

DOI:10.1128/JVI.22.2.273-280.1977

PMID:68124

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515716/

Abstract

An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity. The enzyme had a clear preference for Mg2+ over Mn2+. Cesium sulfate isopycnic gradient centrifugation of the DNA product synthesized in the actinomycin D-containing reaction revealed the presence of DNA-RNA hybrid. Furthermore, the purified DNA product was found to hybridize with RNA isolated from A-particles. These observations strongly indicate that the endogenous A-particle RNA serves as the template for the DNA polymerase.

摘要

发现一种RNA指导的DNA聚合酶与来自DBA/2小鼠白血病细胞的胞质内A颗粒相关。在用2M NaCl-20mM二硫苏糖醇破坏纯化的颗粒后检测到该酶活性。二价阳离子和所有四种脱氧核糖核苷三磷酸的存在对该酶活性至关重要。该酶对Mg2+的偏好明显高于Mn2+。对在含放线菌素D的反应中合成的DNA产物进行硫酸铯等密度梯度离心，结果显示存在DNA-RNA杂交体。此外，发现纯化的DNA产物可与从A颗粒中分离的RNA杂交。这些观察结果有力地表明，内源性A颗粒RNA作为DNA聚合酶的模板。

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本文引用的文献

1

Protein measurement with the Folin phenol reagent.

J Biol Chem. 1951 Nov;193(1):265-75.

2

Electron microscopy of tumor cells and tumor viruses; a review.

Cancer Res. 1958 Jun;18(5):491-509.

3

Noninfectious RSV deficient in DNA polymerase.

Virology. 1971 Jan;43(1):313-6. doi: 10.1016/0042-6822(71)90251-0.

4

DNA polymerase activities in varions of visna virus, a causative agent of a "slow" neurological disease.

Proc Natl Acad Sci U S A. 1971 Jan;68(1):182-6. doi: 10.1073/pnas.68.1.182.

5

Cation preferences for poly(rC)-oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates.

Cancer Res. 1974 Dec;34(12):3509-15.

6

High-molecular-weight DNA associated with intracytoplasmic A-particle inclusions.

Cancer Res. 1974 Oct;34(10):2654-62.

7

Intracytoplasmic A particles: mouse mammary tumor virus nucleoprotein cores?

J Virol. 1973 Apr;11(4):575-84. doi: 10.1128/JVI.11.4.575-584.1973.

8

RNA-directed DNA synthesis and RNA tumor viruses.

Adv Virus Res. 1972;17:129-86. doi: 10.1016/s0065-3527(08)60749-6.

9

Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus.

Biochemistry. 1972 Jun 6;11(12):2334-42. doi: 10.1021/bi00762a020.

10

RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.

Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96. doi: 10.1073/pnas.67.4.1789.

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