抗体多样性分析：四种抗GAT单克隆抗体的V-D-J mRNA核苷酸序列。一种利用交替D-J重组产生功能相似高变区的寡基因系统。
Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of four anti-GAT monoclonal antibodies. A paucigene system using alternate D-J recombinations to generate functionally similar hypervariable regions.
作者信息
Rocca-Serra J, Matthes H W, Kaartinen M, Milstein C, Thèze J, Fougereau M
出版信息
EMBO J. 1983;2(6):867-72. doi: 10.1002/j.1460-2075.1983.tb01515.x.
Abstract
The nucleotide sequence of four anti-(Glu60-Ala30-Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2-terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti-GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D-J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu-Tyr determinant. D regions are encoded by D genes which are closely related either to the D-SP2 or the D.FL16 germ line gene cores.
摘要
测定了四种抗（Glu60 - Ala30 - Tyr10）n（GAT）单克隆γ1重链mRNA从第10密码子到第120密码子的核苷酸序列。该序列与NH2末端氨基酸序列重叠，从而能够阐明包含VH、D和JH区域的完整蛋白质序列。这些高度保守的序列表明，表达相同公共独特型特异性的抗GAT抗体代表一个寡基因系统，该系统使用至少两种D - J组合，导致在识别显性Glu - Tyr决定簇中涉及功能相似的高变区。D区域由与D - SP2或D.FL16种系基因核心密切相关的D基因编码。
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IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.针对磷酸胆碱的IgG抗体比其对应的IgM抗体表现出更多样性。
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Monoclonal anti-GAT antibodies with different fine specificities express the same public idiotype.具有不同精细特异性的单克隆抗GAT抗体表达相同的公用独特型。
Mol Immunol. 1982 Aug;19(8):1001-9. doi: 10.1016/0161-5890(82)90308-x.
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Contribution of the H- and L-chains and of the binding site to the idiotypic specificities of mouse anti-GAT antibodies.重链和轻链以及结合位点对小鼠抗谷氨酸脱羧酶抗体独特型特异性的贡献。
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The immune response of BALB/c mice to phosphorylcholine is restricted to a limited number of VH- and VL-isotypes.BALB/c小鼠对磷酸胆碱的免疫反应仅限于有限数量的重链可变区（VH）和轻链可变区（VL）同种型。
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Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.免疫球蛋白重链多样性DNA片段的组织、结构与组装
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A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.单个VH基因片段编码对磷酸胆碱的免疫反应：体细胞突变与抗体类别相关。
Cell. 1981 Jul;25(1):59-66. doi: 10.1016/0092-8674(81)90231-2.
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