Schiff C, Milili M, Fougereau M
Nucleic Acids Res. 1983 Jun 25;11(12):4007-17. doi: 10.1093/nar/11.12.4007.
A cDNA clone was constructed from a mRNA encoding an anti-GAT (Glu60 Ala30 Tyr10) BALB/c monoclonal antibody heavy chain. Its sequence, covering codons -5 to 162 and therefore encompassing the complete V-D-J region, was determined. Surprisingly, the sequence of the VH gene-encoded region was almost identical with that of the BALB/c VH anti-HNP (4-hydroxy-3-nitrophenyl) acetyl VH region, suggesting that the same VH germ-line might be used to encode two heavy chains contributing to antibodies of discrete specificities. A specific VH probe was derived and annealed to Eco RI and Bg1 II restriction fragments of liver (unrearranged) DNA extracted from the BALB/c, DBA/2 and C57BL/6 mouse strains that differ in their H chain allotypes. Under stringent conditions, only a few bands were identified by Southern blotting. The different patterns observed suggest that the VH anti-GAT repertoire differs between these strains even though their various anti-GAT antibodies express the same public idiotypic specificities.
从编码抗GAT（Glu60 Ala30 Tyr10）BALB/c单克隆抗体重链的mRNA构建了一个cDNA克隆。测定了其序列，该序列覆盖密码子-5至162，因此包含完整的V-D-J区域。令人惊讶的是，VH基因编码区的序列与BALB/c VH抗HNP（4-羟基-3-硝基苯基）乙酰基VH区域的序列几乎相同，这表明相同的VH种系可能用于编码两条重链，它们有助于形成具有不同特异性的抗体。制备了一种特异性VH探针，并将其与从BALB/c、DBA/2和C57BL/6小鼠品系中提取的肝脏（未重排）DNA的Eco RI和Bg1 II限制性片段退火，这些品系的重链同种异型不同。在严格条件下，通过Southern印迹仅鉴定出少数条带。观察到的不同模式表明，尽管这些品系的各种抗GAT抗体表达相同的公共独特型特异性，但它们的VH抗GAT库不同。
