Meshulam T, Verbrugh H A, Verhoef J
Eur J Clin Microbiol. 1982 Apr;1(2):112-7. doi: 10.1007/BF02014202.
The interaction between Pseudomonas aeruginosa strains (6 non-mucoid and 4 mucoid strains), serum factors and phagocytic cells was investigated. Strains were incubated in different concentrations of normal serum, chelated serum (with only the alternative complement pathway intact), IgG, Cls, C2 and C3 deficient serum and immune serum. After incubation complement consumption, C3 fixation and phagocytosis by polymorphonuclear leukocytes (PMN) were measured. In contrast to normal serum, immune serum raised against a mucoid and a non-mucoid strain exhibited heat-stable opsonic activity. All ten Pseudomonas aeruginosa strains were able to activate complement in 20% normal serum, leading to deposition of the activated form of the third complement component on the bacterial cell wall and to subsequent recognition and phagocytosis. One mucoid and four non-mucoid strains activated the alternative complement pathway and were effectively opsonized in chelated or in Cls, C2 or IgG deficient serum. Although mucoid strains were less able to activate complement via the alternative route, no differences were observed in opsonic requirements and phagocytosis between mucoid and non-mucoid strains.
研究了铜绿假单胞菌菌株（6株非黏液型和4株黏液型菌株）、血清因子和吞噬细胞之间的相互作用。将菌株在不同浓度的正常血清、螯合血清（仅替代补体途径完整）、IgG、Cls、C2和C3缺陷血清以及免疫血清中孵育。孵育后，检测补体消耗、C3固定以及多形核白细胞（PMN）的吞噬作用。与正常血清不同，针对黏液型和非黏液型菌株产生的免疫血清表现出热稳定的调理活性。所有十株铜绿假单胞菌菌株都能够在20%的正常血清中激活补体，导致第三种补体成分的活化形式沉积在细菌细胞壁上，并随后被识别和吞噬。一株黏液型菌株和四株非黏液型菌株激活了替代补体途径，并在螯合血清或Cls、C2或IgG缺陷血清中被有效调理。尽管黏液型菌株通过替代途径激活补体的能力较弱，但在黏液型和非黏液型菌株的调理需求和吞噬作用方面未观察到差异。
