Lynn M, Sensakovic J W, Bartell P F
Infect Immun. 1977 Jan;15(1):109-14. doi: 10.1128/iai.15.1.109-114.1977.
Intraperitoneal injection of slime glycolipoprotein (GLP) from Pseudomonas aeruginosa induced leukopenia and death of mice, similar to the effect of infection with viable organisms. Differential counts established that the leukopenia was characterized by a decrease in the number of polymorphonuclear leukocytes, followed by death of mice. Mice immunized with GLP survived challenge and responded with a leukocytosis that had a substantial increase in circulating polymorphonuclear leukocytes. Leukocytes from GLP-injected mice were agglutinated by anti-GLP serum, indicating an association between GLP and leukocytes. Other results indicated that 14C-labeled GLP is deposited mainly in the liver. Normal leukocytes labeled with 51Cr were injected intravenously into mice receiving an intraperitoneal injection of GLP. As with GLP, the 51Gr-labeled leukocytes were sequestered in the liver. These results indicate that GLP enters the blood stream and becomes associated mainly with neutrophils, and that the neutrophil-GLP complex is deposited in the liver, possibly accounting for the leukopenia in mice.
腹腔注射铜绿假单胞菌的黏液糖脂蛋白（GLP）可导致小鼠白细胞减少和死亡，这与感染活的细菌的效果相似。分类计数表明，白细胞减少的特征是多形核白细胞数量减少，随后小鼠死亡。用GLP免疫的小鼠在受到攻击后存活下来，并出现白细胞增多，循环中的多形核白细胞大幅增加。注射GLP的小鼠的白细胞被抗GLP血清凝集，表明GLP与白细胞之间存在关联。其他结果表明，14C标记的GLP主要沉积在肝脏中。将用51Cr标记的正常白细胞静脉注射到接受腹腔注射GLP的小鼠体内。与GLP一样，51Cr标记的白细胞被隔离在肝脏中。这些结果表明，GLP进入血流并主要与中性粒细胞结合，并且中性粒细胞 - GLP复合物沉积在肝脏中，这可能是小鼠白细胞减少的原因。
