Sá-Nogueira I, de Lencastre H
Laboratório de Genetica Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
J Bacteriol. 1989 Jul;171(7):4088-91. doi: 10.1128/jb.171.7.4088-4091.1989.
Two recombinant plasmids, pSNL1 and pSNL2, carrying structural genes for L-arabinose utilization were isolated from a Bacillus subtilis gene library. Both plasmids complemented araD mutations in a Rec- B. subtilis strain and in Escherichia coli. Moreover, pSNL1 also complemented araB mutations in both species and efficiently transformed araA Rec+ B. subtilis strains to Ara+. Detailed physical mapping of both plasmids in addition to transformation experiments involving defined restriction fragments from the pSNL1 insert unambiguously determined the gene order to be araD, araB, and araA, an order different from that found in E. coli.
从枯草芽孢杆菌基因文库中分离出两个携带L-阿拉伯糖利用结构基因的重组质粒pSNL1和pSNL2。这两个质粒均能互补Rec⁻枯草芽孢杆菌菌株和大肠杆菌中的araD突变。此外，pSNL1还能互补这两个物种中的araB突变，并能有效地将araA Rec⁺枯草芽孢杆菌菌株转化为Ara⁺。除了涉及pSNL1插入片段中特定限制酶切片段的转化实验外，对这两个质粒进行的详细物理图谱分析明确确定了基因顺序为araD、araB和araA，这一顺序与在大肠杆菌中发现的不同。
