Masuda Y, Yoshimura H
J Toxicol Sci. 1982 Aug;7(3):161-75. doi: 10.2131/jts.7.161.
A typical lot of Kanemi rice oil ingested by patients with yusho (PCB poisoning) and the blood, liver and adipose tissue of the patients were analyzed for individual congeners of polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) by gas chromatography and gas chromatography-mass spectrometry. The individual congeners identified were assayed for biological properties such as accumulation ability in the liver of monkeys and rats, inducing activities of benzo[a]pyrene 3-hydroxylase, benzphetamine demethylase and DT diaphorase in rats, and gravimetric changes of the thymus and liver in rats. Among the seven PCB congeners detected in yusho patients, 2, 3, 4, 5, 3', 4'-hexa-CB seems to be the most related compound to yusho by its strong effects on induction of the liver enzymes, and on atrophy of the thymus and hypertrophy of the liver in rats. PCDF congeners identified in the patients showed severe toxicity in rats than this PCB, exhibiting stronger enzyme induction and gravimetric changes of the tissues even at very low doses of 1-10 micrograms/kg. These PCDFs, especially 2, 3, 4, 7, 8-penta-CDF, were also very accumulative in the liver. Therefore, they are considered as the most important etiologic agents for current symptoms of yusho.
对患有油症（多氯联苯中毒）的患者摄入的一批典型的日本米糠油，以及患者的血液、肝脏和脂肪组织，通过气相色谱法和气相色谱 - 质谱分析法分析了多氯联苯（PCBs）和多氯二苯并呋喃（PCDFs）的各个同系物。对鉴定出的各个同系物进行了生物学特性测定，如在猴子和大鼠肝脏中的蓄积能力、在大鼠中诱导苯并[a]芘3 - 羟化酶、苄非他明脱甲基酶和DT黄递酶的活性，以及大鼠胸腺和肝脏的重量变化。在油症患者体内检测到的7种多氯联苯同系物中，2,3,4,5,3',4'-六氯联苯因其对大鼠肝脏酶诱导、胸腺萎缩和肝脏肥大有强烈影响，似乎是与油症关系最密切的化合物。在患者体内鉴定出的多氯二苯并呋喃同系物在大鼠中显示出比这种多氯联苯更严重的毒性，即使在1 - 10微克/千克的极低剂量下也表现出更强的酶诱导和组织重量变化。这些多氯二苯并呋喃，尤其是2,3,4,7,8 - 五氯二苯并呋喃，在肝脏中也非常容易蓄积。因此，它们被认为是导致油症当前症状的最重要病因。
