巨大芽孢杆菌的代谢与萌发触发。利用L-[3H]丙氨酸和氚水检测代谢。
Metabolism and the triggering of germination of Bacillus megaterium. Use of L-[3H]alanine and tritiated water to detect metabolism.
作者信息
Scott I R, Ellar D J
出版信息
Biochem J. 1978 Aug 15;174(2):635-40. doi: 10.1042/bj1740635.
Abstract
L-[2,3-3H]Alanine was used to probe for metabolism of alanine during triggering of germination of spores of Bacillus megaterium KM. No detectable incorporation of label into any compound, including water, was found, indicating that any metabolism involving the alanine germinant must be at a very low rate and also that alanine racemase is absent from spores of this strain. Spores were germinated in 3H2O to find if any of the many metabolic reactions causing irreversible incorporation of 3H into reaction products took place during triggering of germination. No incorporation was detected until 2-3 min after addition of germinants. It is therefore concluded that a wide variety of metabolic routes, including glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and amino acid metabolism are either not involved in the reactions causing the triggering of germination or operate at an extremely low rate during this process.
摘要
L-[2,3-³H]丙氨酸被用于探究巨大芽孢杆菌KM菌株孢子萌发触发过程中丙氨酸的代谢情况。未发现标记物掺入任何化合物（包括水），这表明涉及丙氨酸萌发剂的任何代谢速率都非常低，并且该菌株的孢子中不存在丙氨酸消旋酶。将孢子在³H₂O中萌发，以确定在萌发触发过程中是否发生了许多导致³H不可逆掺入反应产物的代谢反应。在添加萌发剂后2 - 3分钟之前未检测到掺入。因此可以得出结论，包括糖酵解、三羧酸循环、磷酸戊糖途径和氨基酸代谢在内的多种代谢途径，要么不参与引发萌发的反应，要么在此过程中以极低的速率运行。
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本文引用的文献
1
Water Permeability of Bacterial Spores and the Concept of a Contractile Cortex.细菌孢子的透水性和收缩皮层的概念。
Science. 1960 Aug 26;132(3426):544-5. doi: 10.1126/science.132.3426.544.
2
Intermediate metabolism of aerobic spores. IV. Alanine deamination during the germination of spores of Bacillus cereus.需氧芽孢的中间代谢。IV. 蜡样芽孢杆菌芽孢萌发过程中的丙氨酸脱氨基作用。
J Bacteriol. 1959 Dec;78(6):844-51. doi: 10.1128/jb.78.6.844-851.1959.
3
Studies on the role of L-alanine in the germination of spores of Bacillus terminalis.关于L-丙氨酸在破伤风芽孢杆菌孢子萌发中作用的研究。
J Bacteriol. 1955 Mar;69(3):275-9. doi: 10.1128/jb.69.3.275-279.1955.
4
ROLE OF AMINO ACIDS IN GERMINATION OF BACILLUS LICHENIFORMIS SPORES. I. UPTAKE OF RADIOACTIVE AMINO ACIDS BY GERMINATING AND DORMANT SPORES.
J Dairy Sci. 1965 Mar;48:282-6. doi: 10.3168/jds.s0022-0302(65)88217-0.
5
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Proc Natl Acad Sci U S A. 1964 Jun;51(6):1164-72. doi: 10.1073/pnas.51.6.1164.
6
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Ann Inst Pasteur (Paris). 1970 May;118(5):611-25.
7
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8
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J Bacteriol. 1972 Apr;110(1):321-8. doi: 10.1128/jb.110.1.321-328.1972.
9
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10
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J Appl Bacteriol. 1970 Mar;33(1):34-49. doi: 10.1111/j.1365-2672.1970.tb05232.x.
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