Scott I R, Ellar D J
Biochem J. 1978 Aug 15;174(2):635-40. doi: 10.1042/bj1740635.
L-[2,3-3H]Alanine was used to probe for metabolism of alanine during triggering of germination of spores of Bacillus megaterium KM. No detectable incorporation of label into any compound, including water, was found, indicating that any metabolism involving the alanine germinant must be at a very low rate and also that alanine racemase is absent from spores of this strain. Spores were germinated in 3H2O to find if any of the many metabolic reactions causing irreversible incorporation of 3H into reaction products took place during triggering of germination. No incorporation was detected until 2-3 min after addition of germinants. It is therefore concluded that a wide variety of metabolic routes, including glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and amino acid metabolism are either not involved in the reactions causing the triggering of germination or operate at an extremely low rate during this process.
L-[2,3-³H]丙氨酸被用于探究巨大芽孢杆菌KM菌株孢子萌发触发过程中丙氨酸的代谢情况。未发现标记物掺入任何化合物（包括水），这表明涉及丙氨酸萌发剂的任何代谢速率都非常低，并且该菌株的孢子中不存在丙氨酸消旋酶。将孢子在³H₂O中萌发，以确定在萌发触发过程中是否发生了许多导致³H不可逆掺入反应产物的代谢反应。在添加萌发剂后2 - 3分钟之前未检测到掺入。因此可以得出结论，包括糖酵解、三羧酸循环、磷酸戊糖途径和氨基酸代谢在内的多种代谢途径，要么不参与引发萌发的反应，要么在此过程中以极低的速率运行。
