Löning T, Caselitz J, Seifert G, Weber K, Osborn M
Virchows Arch A Pathol Anat Histopathol. 1982;398(2):119-28. doi: 10.1007/BF00618863.
Langerhans cells in oral mucosa and epidermis have been identified using antibodies to intermediate filament proteins in conjunction with antibodies to T6 and HLA-DR antigens. Langerhans cells, lymphocytes and melanocytes are positive when tested with antibodies to vimentin, and negative with antibodies to prekeratin. Langerhans cells are also positive with antibodies directed against HLA-DR and T6 antigens. In contrast keratinocytes are positive for prekeratin and negative for vimentin, HLA-DR and T6. These methods provide a useful tool for the identification of Langerhans cells at the level of the light microscope, and have been used to show changes in the number and arrangement of these cells in squamous cell carcinomas of skin and oral mucosa, and in inflammatory conditions.
利用针对中间丝蛋白的抗体结合针对T6和HLA - DR抗原的抗体，已在口腔黏膜和表皮中鉴定出朗格汉斯细胞。用波形蛋白抗体检测时，朗格汉斯细胞、淋巴细胞和黑素细胞呈阳性，而用前角蛋白抗体检测时呈阴性。针对HLA - DR和T6抗原的抗体检测，朗格汉斯细胞也呈阳性。相比之下，角质形成细胞前角蛋白呈阳性，波形蛋白、HLA - DR和T6呈阴性。这些方法为在光学显微镜水平鉴定朗格汉斯细胞提供了有用的工具，并已用于显示皮肤和口腔黏膜鳞状细胞癌以及炎症状态下这些细胞数量和排列的变化。
