de Waal R M, Semeijn J T, Cornelissen M H, Ramaekers F C
J Invest Dermatol. 1984 Jun;82(6):602-4. doi: 10.1111/1523-1747.ep12261429.
In epidermal cell suspensions, prepared from healthy human and murine skin, Langerhans cells (LC) were identified by indirect immunofluorescence microscopy using monoclonal antibodies directed against human T6 and Ia markers, and against a murine Iak determinant, respectively. The cells were double-labeled on cytospin slides with antibodies against a cytoskeletal protein, either vimentin or keratin. In this way it was shown that epidermal LC contain intermediate-sized filaments of the vimentin type.
在从健康人类和小鼠皮肤制备的表皮细胞悬液中，分别使用针对人类T6和Ia标志物以及针对小鼠Iak决定簇的单克隆抗体，通过间接免疫荧光显微镜鉴定朗格汉斯细胞（LC）。将细胞在细胞离心涂片载玻片上用针对细胞骨架蛋白波形蛋白或角蛋白的抗体进行双重标记。通过这种方式表明，表皮LC含有波形蛋白类型的中等大小细丝。
