Caselitz J, Jänner M, Breitbart E, Weber K, Osborn M
Virchows Arch A Pathol Anat Histopathol. 1983;400(1):43-51. doi: 10.1007/BF00627007.
Six malignant melanomas have been examined for the type of intermediate filament they contain. All six cases showed positive staining of intermediate filaments with antibodies to vimentin, with cells containing large numbers of melanosomes being stained less strongly in general. The tumor cells did not react with antibodies to keratin, desmin, neurofilaments or glial fibrillary acidic protein. Thus typing of intermediate filaments can distinguish melanoma from undifferentiated carcinoma, but not from lymphoma or sarcoma. Since melanocytes are known to be vimentin positive, and since most of the samples we studied were from metastases, these results are a further indication that the intermediate filament type typical of the parental cell is retained in the metastases, as well as in the primaries of solid tumours. The implications of vimentin positivity for the histiogenesis of the melanocyte are also discussed.
对六个恶性黑色素瘤样本进行了检查，以确定它们所含中间丝的类型。所有六个病例的中间丝与波形蛋白抗体呈阳性染色，一般来说，含有大量黑素小体的细胞染色较弱。肿瘤细胞与角蛋白、结蛋白、神经丝或胶质纤维酸性蛋白抗体均无反应。因此，中间丝分型可将黑色素瘤与未分化癌区分开来，但不能与淋巴瘤或肉瘤区分。由于已知黑素细胞波形蛋白呈阳性，且我们研究的大多数样本来自转移灶，这些结果进一步表明，亲代细胞典型的中间丝类型在转移灶以及实体瘤的原发灶中均得以保留。本文还讨论了波形蛋白阳性对黑素细胞组织发生的影响。
