Otomo S, Nakaike S, Tsuji I, Mori C, Ohzeki M
Nihon Yakurigaku Zasshi. 1981 Mar;77(3):313-20.
D-penicillamine (D-PA) has beneficial therapeutic effects for patients with rheumatoid arthritis but no convincing explanation has been offered for the mode of action. Experiments reported here were designed to gain an insight into the related mechanisms. Wistar rats were inoculated with various doses of Mycobacterium tuberculosis to induce adjuvant arthritis, and on the 21st day, the lesions of paws and ears were graded according to the extent of the erythema and swelling. Rats given D-PA simultaneously with the inoculation of M. tuberculosis developed a more severe arthritis than that seen in the control group, when they were inoculated with low doses of M. tuberculosis. To investigate the effect of D-PA on hemolytic plaque forming cells (PFC) in the spleen, BDF1 mice were immunized with various doses of sheep red blood cells (SRBC) and D-PA was injected in various doses and at various times. D-PA produced either enhancement or depression of the number of PFC, depending on the dose of antigenic stimulus of SRBC. Furthermore, D-PA slightly enhanced the concanavalin A-induced blastogenesis of the spleen cells in vitro, at a concentration of 1-50 microM, but at concentrations of 500 microM, inhibition was evident. These results indicate that D-PA may act as an immunomodulating agent.
D-青霉胺（D-PA）对类风湿性关节炎患者具有有益的治疗作用，但尚未对其作用方式给出令人信服的解释。本文报道的实验旨在深入了解相关机制。给Wistar大鼠接种不同剂量的结核分枝杆菌以诱发佐剂性关节炎，并在第21天根据红斑和肿胀程度对爪部和耳部病变进行分级。当接种低剂量结核分枝杆菌时，与接种结核分枝杆菌同时给予D-PA的大鼠发生的关节炎比对照组更严重。为了研究D-PA对脾脏中溶血空斑形成细胞（PFC）的影响，用不同剂量的绵羊红细胞（SRBC）免疫BDF1小鼠，并在不同时间注射不同剂量的D-PA。D-PA对PFC数量的影响取决于SRBC抗原刺激的剂量，可产生增强或抑制作用。此外，在浓度为1 - 50微摩尔时，D-PA在体外可轻微增强伴刀豆球蛋白A诱导的脾细胞增殖，但在浓度为500微摩尔时，抑制作用明显。这些结果表明D-PA可能作为一种免疫调节剂发挥作用。
