Sáez-Nieto J A, Fenoll A, Vazquez J, Casal J
J Clin Microbiol. 1982 Jan;15(1):78-81. doi: 10.1128/jcm.15.1.78-81.1982.
We studied the prevalence of maltose-negative variants of Neisseria meningitidis in Spain from 1978 to 1980. Sugar utilization studies were performed with both CTA medium and Mueller-Hinton medium; bromothymol blue was used as the indicator in Mueller-Hinton medium. Of 1,714 isolates of N. meningitidis recovered from the cerebrospinal fluid or blood of patients with meningococcal infections, 64 (3.7%) were maltose-negative variants; 13 (3.3%) of the 363 isolates found in carriers had the same characteristic. All maltose-negative cultures isolated from both patients and carriers belonged to serogroup B and were resistant to sulfadiazine at a minimal inhibitory concentration, 10 micrograms/ml or more. Serotype 2 isolates were the most prevalent isolates in patients (68.8%), followed by nontypable isolates (20.3%). Only serotype 2 isolates (66%) and nontypable isolates (33%) were found in carriers.
我们研究了1978年至1980年西班牙脑膜炎奈瑟菌麦芽糖阴性变体的流行情况。使用CTA培养基和Mueller-Hinton培养基进行糖利用研究；在Mueller-Hinton培养基中使用溴百里酚蓝作为指示剂。从脑膜炎球菌感染患者的脑脊液或血液中分离出的1714株脑膜炎奈瑟菌中，64株（3.7%）为麦芽糖阴性变体；在携带者中发现的363株分离株中有13株（3.3%）具有相同特征。从患者和携带者中分离出的所有麦芽糖阴性培养物均属于B血清群，对磺胺嘧啶的最低抑菌浓度为10微克/毫升或更高时具有抗性。2型菌株是患者中最常见的分离株（68.8%），其次是不可分型分离株（20.3%）。在携带者中仅发现2型菌株（66%）和不可分型分离株（33%）。
